| Detailed Information for Phage Saal |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sahil Aggarwal |
| Year Found | 2011 |
| Location Found | Richmond, VA USA |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.548611 N, 77.449575 W Map |
| Discovery Notes | The phage was discovered in moist soil at a depth or approximately 3 inches. The outside air temperature was approximately 75 degrees. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 18, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 184 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 57775 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | A typical plaque is clear and relatively smooth. In terms of size, there are both small (approximately 0.5mm) and large (approximately 1.5mm) plaques in a single plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ025956 |
| Refseq Number | NC_023580 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011VACUSaalSA |
| SEA Lysate Titer |
9.7e14 |
| Date of SEA Lysate Titering |
Oct 19, 2011 |
| Pitt Freezer Box# |
30 |
| Pitt Freezer Box Grid# |
63 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |