The Actinobacteriophage Database

Mycobacterium phage Scottish

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Detailed Information for Phage Scottish
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Olivia Cummings
Year Found	2015
Location Found	Providence, RI USA
Finding Institution	Brown University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	41.824167 N, 71.403333 W Map
Discovery Notes	Scottish was found on 9/11/2015 in moist soil (it had rained the day before). The soil was located in Keeney Quad, about 2cm under a tree.
Naming Notes	The unofficial name of Scottish is "The Scottish Phage," which is a play on a superstitious name for Shakespeare's "Macbeth," "The Scottish Play." Like "Macbeth," Scottish (the phage) is quick, aggressive, and does not turn out well (for M. Smeg in this case).
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 8, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	1041
Genome length (bp)	56825
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	Rockland Community College
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	105
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Scottish_1 (terminase small subunit) Scottish_2 (terminase large subunit) Scottish_3 (portal protein) Scottish_4 (capsid maturation protease) Scottish_5 (scaffolding protein) Scottish_6 (major capsid protein) Scottish_7 (head-to-tail adaptor) Scottish_8 (head-to-tail stopper) Scottish_9 Scottish_10 (tail terminator) Scottish_11 (major tail protein) Scottish_12 (tail assembly chaperone) Scottish_13 (tail assembly chaperone) Scottish_14 (tape measure protein) Scottish_15 (transposase) Scottish_16 (minor tail protein) Scottish_17 (minor tail protein) Scottish_18 (minor tail protein) Scottish_19 (minor tail protein) Scottish_20 (minor tail protein) Scottish_21 (minor tail protein) Scottish_22 Scottish_23 Scottish_24 Scottish_25 Scottish_26 (helix-turn-helix DNA-binding domain protein) Scottish_27 Scottish_28 Scottish_29 Scottish_30 Scottish_31 (lysin A) Scottish_32 (lysin B) Scottish_33 Scottish_34 Scottish_35 Scottish_36 (DnaQ-like DNA polymerase III subunit) Scottish_37 Scottish_38 Scottish_39 Scottish_40 Scottish_41 Scottish_42 Scottish_43 (tyrosine integrase) Scottish_44 Scottish_45 Scottish_46 (immunity repressor) Scottish_47 (Cro protein) Scottish_48 Scottish_49 (excise) Scottish_50 Scottish_51 Scottish_52 Scottish_53 Scottish_54 Scottish_55 (WhiB family transcription factor) Scottish_56 (helix-turn-helix DNA-binding domain protein) Scottish_57 (WhiB family transcription factor) Scottish_58 (helix-turn-helix DNA-binding domain protein) Scottish_59 Scottish_60 Scottish_61 Scottish_62 (RecA-like DNA recombinase) Scottish_63 Scottish_64 (HNH endonuclease) Scottish_65 Scottish_66 Scottish_67 (DnaQ-like DNA polymerase III subunit) Scottish_68 (DNA methylase) Scottish_69 (DNA methylase) Scottish_70 Scottish_71 Scottish_72 (DNA methylase) Scottish_73 Scottish_74 Scottish_75 Scottish_76 Scottish_77 Scottish_78 Scottish_79 Scottish_80 Scottish_81 Scottish_82 Scottish_83 Scottish_84 Scottish_85 Scottish_86 Scottish_87 Scottish_88 Scottish_89 Scottish_90 Scottish_91 Scottish_92 Scottish_93 Scottish_94 (MerR-like helix-turn-helix DNA binding domain protein) Scottish_95 Scottish_96 Scottish_97 Scottish_98 Scottish_99 Scottish_100 Scottish_101 (glycosyltransferase) Scottish_102 Scottish_103 Scottish_104 (glycosyltransferase) Scottish_105 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN735433
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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