| Detailed Information for Phage Seagreen |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Carlyle Seagreen |
| Year Found | 2014 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.817338 S, 30.944967 E Map |
| Discovery Notes | Soil sample was taken from a flower bed wherein the soil was relatively dry. The sample was enriched, after which the phages were seen. Taken from the surface of the soil. |
| Naming Notes | The name was taken from the finders surname. (Seagreen) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 21, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2910 |
| Genome length (bp) | 57766 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCGC |
| GC Content | 61.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques have an inner circle that is clear, measuring 2 millimeters. Aftr 36-48 hours the plaques form a clear halo. Including the halo the plaque measures 5mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT281793 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
