Detailed Information for Phage Seagreen |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Carlyle Seagreen |
Year Found | 2014 |
Location Found | Durban, South Africa |
Finding Institution | University of Kwazulu-Natal |
Program | Mycobacterial Genetics Course, Durban, South Africa |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 29.817338 S, 30.944967 E Map |
Discovery Notes | Soil sample was taken from a flower bed wherein the soil was relatively dry. The sample was enriched, after which the phages were seen. Taken from the surface of the soil. |
Naming Notes | The name was taken from the finders surname. (Seagreen) |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | May 21, 2015 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 2910 |
Genome length (bp) | 57766 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGTAGGCGC |
GC Content | 61.8% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | The plaques have an inner circle that is clear, measuring 2 millimeters. Aftr 36-48 hours the plaques form a clear halo. Including the halo the plaque measures 5mm. |
Morphotype | Siphoviridae |
Number of Genes | 105 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KT281793 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
4 |
Pitt Freezer Box Grid# |
D2 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |