| Detailed Information for Phage Soul22 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Sol22, Revolution22 |
| Found By | Alexis Koehl |
| Year Found | 2019 |
| Location Found | Holland, MI United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.7697 N, 86.1664 W Map |
| Discovery Notes | Collected on September 12th from compost pile at 8:30 am.
Moved away newer fresh compost and collected underneath.
Heavy rain the night before |
| Naming Notes | Named because my phage morphology has several halos and it reminded me of the sun rays that form halos around the sun and those are called 22-degree halos. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 19, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 876 |
| Genome length (bp) | 54814 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCAGGGCAT |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | The phage is oval shaped and has a turbid middle with a clear ring around it and it has a turbid ring around it and a turbid tail. It is less than .5 cm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT522000 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
100 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
