| Detailed Information for Phage Squirty |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Erin Osborne |
| Year Found | 2012 |
| Location Found | Belleville, MI USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.19 N, 83.5 W Map |
| Discovery Notes | It is from mulch in a garden in my front years. It was from about an inch underground. The soil was dry. The temperature that day was 84 degrees Fahrenheit and was 90% humidity. |
| Naming Notes | It was my nickname as a child. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 30, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 430 |
| Sanger Finishing Reactions | 11 |
| Genome length (bp) | 60285 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGACGGCAT |
| GC Content | 62.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaque was small and clear. It was always about 1mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM101124 |
| Refseq Number | NC_026588 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
38 |
| Pitt Freezer Box Grid# |
14 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
