| Detailed Information for Phage Tchotchke |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lizzet Arellano & Dinh Huynh |
| Year Found | 2023 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 109°C |
| GPS Coordinates | 36.113664 N, 115.143897 W Map |
| Naming Notes | I wanted something Yiddish because a normal English name wasn't appealing to me. Tchotcke means "thing" in Yiddish which is what the bacteriophage is! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2025 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 108570 |
| Genome length (bp) | 55682 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | initially, it was lysogenic and then it became lytic. |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | Being Annotated (Expected completion by 12/31/2025) |
| Plaque Notes | small plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
183 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |