Mycobacterium phage ThetaBob
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Detailed Information for Phage ThetaBob
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNathan Terry
Year Found2014
Location FoundHot Springs, AR USA
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.458881 N, 93.02645 W Map
Naming NotesWanted to have a greek letter
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 27, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage821
Genome length (bp)56714
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGATGGCTT
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF4
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionOuachita Baptist University
Annotation StatusIn GenBank
Plaque NotesThe phage is Lysogenic with a turbid plaque. Plaques are primarily large with some smaller plaques (see plaque picture).
MorphotypeSiphoviridae
Number of Genes106
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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ThetaBob_1

ThetaBob_2
(terminase)

ThetaBob_3
(portal protein)

ThetaBob_4
(capsid maturation protease)

ThetaBob_5
(scaffolding protein)

ThetaBob_6

ThetaBob_7
(major capsid protein)

ThetaBob_8

ThetaBob_9
(head-to-tail connector complex protein)

ThetaBob_10

ThetaBob_11
(head-to-tail connector complex protein)

ThetaBob_12
(major tail protein)

ThetaBob_13
(tail assembly chaperone)

ThetaBob_14
(tail assembly chaperone)

ThetaBob_15
(tape measure protein)

ThetaBob_16
(minor tail protein)

ThetaBob_17
(minor tail protein)

ThetaBob_18
(minor tail protein)

ThetaBob_19
(minor tail protein)

ThetaBob_20
(minor tail protein)

ThetaBob_21
(minor tail protein)

ThetaBob_22
(minor tail protein)

ThetaBob_23

ThetaBob_24
(helix-turn-helix DNA binding domain protein)

ThetaBob_25

ThetaBob_26

ThetaBob_27

ThetaBob_28

ThetaBob_29
(lysin A)

ThetaBob_30
(lysin B)

ThetaBob_31

ThetaBob_32

ThetaBob_33

ThetaBob_34

ThetaBob_35

ThetaBob_36

ThetaBob_37

ThetaBob_38

ThetaBob_39

ThetaBob_40

ThetaBob_41

ThetaBob_42

ThetaBob_43

ThetaBob_44

ThetaBob_45
(tyrosine integrase)

ThetaBob_46
(G-I-Y Y-I-G endonuclease)

ThetaBob_47

ThetaBob_48

ThetaBob_49
(helix-turn-helix DNA binding domain protein)

ThetaBob_50

ThetaBob_51

ThetaBob_52

ThetaBob_53

ThetaBob_54

ThetaBob_55

ThetaBob_56

ThetaBob_57

ThetaBob_58

ThetaBob_59

ThetaBob_60

ThetaBob_61
(WhiB family transcription factor)

ThetaBob_62
(helix-turn-helix DNA binding domain protein)

ThetaBob_63

ThetaBob_64
(WhiB family transcription factor)

ThetaBob_65
(helix-turn-helix DNA binding domain protein)

ThetaBob_66

ThetaBob_67

ThetaBob_68

ThetaBob_69

ThetaBob_70

ThetaBob_71
(AAA-ATPase)

ThetaBob_72

ThetaBob_73
(HNH endonuclease)

ThetaBob_74

ThetaBob_75
(DNA methylase)

ThetaBob_76

ThetaBob_77
(G-I-Y Y-I-G endonuclease)

ThetaBob_78

ThetaBob_79

ThetaBob_80

ThetaBob_81

ThetaBob_82
(MPME1 protein)

ThetaBob_83

ThetaBob_84

ThetaBob_85

ThetaBob_86

ThetaBob_87

ThetaBob_88

ThetaBob_89

ThetaBob_90

ThetaBob_91

ThetaBob_92

ThetaBob_93

ThetaBob_94

ThetaBob_95

ThetaBob_96

ThetaBob_97

ThetaBob_98

ThetaBob_99

ThetaBob_100

ThetaBob_101

ThetaBob_102

ThetaBob_103
(glycosyltransferase)

ThetaBob_104
(galactosyltransferase)

ThetaBob_105
(glycosyltransferase)

ThetaBob_106

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK977709
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.4e10pfu/ml
Pitt Freezer Box# 8
Pitt Freezer Box Grid# E10
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload