| Detailed Information for Phage Topiatin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Martin Uwayo Nkomeje |
| Year Found | 2025 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Unavailable |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.052916 S, 18.476544 E Map |
| Discovery Notes | Found in the garden just post heavy rain and before continued heavy rain. Walked outside, dug the soil, and discovered the sample /phage. The soil was rich, in the past plenty of crops grew there, indicating a fertile environment. |
| Naming Notes | The name "Topiatin" is derived from the Greek concept of "topia," which relates to "place" or "location" (inspired by the idea of an ideal place or utopia). Since the name "Utopia" was already taken, the "top" from "topia" was kept, and "tin" comes from the last three letters of my name, Martin. The name symbolizes a phage that would be of utility, helping lead us closer to a utopian society while marking my personal involvement in the discovery. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 11, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 164 |
| Genome length (bp) | 59361 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCGC |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | Being Annotated (Expected completion by 2/11/2026) |
| Has been Phamerated? | No |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
