| Detailed Information for Phage Veteran |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Laura Shellooe |
| Year Found | 2012 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 47.66805 N, 117.400492 W Map |
| Discovery Notes | Date: 9-14-12
Time: 17:30
Location: Gonzaga University campus,flower bed, flat and not close to foot traffic
Depth: 3cm
Soil Moisture: moist soil
Temp: 28°C or 82°F |
| Naming Notes | The soil that the phage was isolated from was located in a garden planted to take the shape and design of the American flag. It was this and the fact that this phage survived the many dilutions and processes of isolation, that I named this phage Veteran. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 16, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 461 |
| Genome length (bp) | 56147 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology was observed as showing plaques up to 3mm in diameter, depending on the concentration of plaques on the agar plates. The plaques had defined parameters with a cleared center and a build-up of bacteria around the edges of the plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT114164 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012GONZveteranLTS |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
14 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
