| Detailed Information for Phage Violac |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Amber Vosloo |
| Year Found | 2024 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.958114 S, 18.457455 E Map |
| Discovery Notes | The phage was found in a compost heap at the University of Cape Town. The soil was harvested approximately 10 cm from the surface. |
| Naming Notes | The student combined the words "violet" and "lilac"; their favourite colour is purple. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3570 |
| Genome length (bp) | 58196 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were clear. With higher confluency, the plaques had a diameter of less than 1 mm.
As the phage plates became more diluted, the plaque diameter increased to approximately 4 mm, due to the presence of plaque halos. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV755394 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |