Mycobacterium phage BuzzLyseyear
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Detailed Information for Phage BuzzLyseyear
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMatthew Walton
Year Found2013
Location FoundColumbus, OH USA
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.001674 N, 83.013416 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 7, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage49
Sanger Finishing Reactions10
Genome length (bp)59419
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGAAGGCAT
GC Content61.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes1 mm diameter, cloudy
MorphotypeSiphoviridae
Number of Genes110
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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BuzzLyseyear_1
(HNH endonuclease)

BuzzLyseyear_2
(terminase small subunit)

BuzzLyseyear_3
(terminase large subunit)

BuzzLyseyear_4
(portal protein)

BuzzLyseyear_5
(capsid maturation protease)

BuzzLyseyear_6
(scaffolding protein)

BuzzLyseyear_7
(major capsid protein)

BuzzLyseyear_8
(head-to-tail adaptor)

BuzzLyseyear_9
(head-to-tail stopper)

BuzzLyseyear_10

BuzzLyseyear_11
(tail terminator)

BuzzLyseyear_12
(major tail subunit)

BuzzLyseyear_13
(tail assembly chaperone)

BuzzLyseyear_14
(tail assembly chaperone)

BuzzLyseyear_15
(HNH endonuclease)

BuzzLyseyear_16
(tape measure protein)

BuzzLyseyear_17
(minor tail protein)

BuzzLyseyear_18
(minor tail protein)

BuzzLyseyear_19
(minor tail protein)

BuzzLyseyear_20
(minor tail protein)

BuzzLyseyear_21
(D-ala-D-ala carboxypeptidase)

BuzzLyseyear_22
(minor tail protein)

BuzzLyseyear_23

BuzzLyseyear_24

BuzzLyseyear_25

BuzzLyseyear_26

BuzzLyseyear_27

BuzzLyseyear_28

BuzzLyseyear_29

BuzzLyseyear_30

BuzzLyseyear_31

BuzzLyseyear_32

BuzzLyseyear_33
(lysin A)

BuzzLyseyear_34
(lysin B)

BuzzLyseyear_35
(holin)

BuzzLyseyear_36

BuzzLyseyear_37

BuzzLyseyear_38
(DnaQ-like DNA polymerase III subunit)

BuzzLyseyear_39

BuzzLyseyear_40

BuzzLyseyear_41

BuzzLyseyear_42

BuzzLyseyear_43

BuzzLyseyear_44

BuzzLyseyear_45

BuzzLyseyear_46
(HTH DNA binding protein)

BuzzLyseyear_47

BuzzLyseyear_48
(tyrosine integrase)

BuzzLyseyear_49

BuzzLyseyear_50
(immunity repressor)

BuzzLyseyear_51

BuzzLyseyear_52
(antirepressor)

BuzzLyseyear_53

BuzzLyseyear_54

BuzzLyseyear_55
(DNA binding protein)

BuzzLyseyear_56

BuzzLyseyear_57

BuzzLyseyear_58

BuzzLyseyear_59

BuzzLyseyear_60

BuzzLyseyear_61

BuzzLyseyear_62

BuzzLyseyear_63
(WhiB)

BuzzLyseyear_64

BuzzLyseyear_65
(WhiB)

BuzzLyseyear_66
(HTH DNA binding protein)

BuzzLyseyear_67

BuzzLyseyear_68

BuzzLyseyear_69

BuzzLyseyear_70
(AAA-ATPase)

BuzzLyseyear_71
(HNH endonuclease)

BuzzLyseyear_72
(DNA methylase)

BuzzLyseyear_73

BuzzLyseyear_74

BuzzLyseyear_75

BuzzLyseyear_76

BuzzLyseyear_77

BuzzLyseyear_78

BuzzLyseyear_79

BuzzLyseyear_80

BuzzLyseyear_81

BuzzLyseyear_82

BuzzLyseyear_83

BuzzLyseyear_84

BuzzLyseyear_85

BuzzLyseyear_86

BuzzLyseyear_87

BuzzLyseyear_88

BuzzLyseyear_89

BuzzLyseyear_90

BuzzLyseyear_91

BuzzLyseyear_92

BuzzLyseyear_93

BuzzLyseyear_94

BuzzLyseyear_95

BuzzLyseyear_96

BuzzLyseyear_97

BuzzLyseyear_98

BuzzLyseyear_99

BuzzLyseyear_100

BuzzLyseyear_101
(putative transcriptional regulator)

BuzzLyseyear_102

BuzzLyseyear_103

BuzzLyseyear_104

BuzzLyseyear_105

BuzzLyseyear_106

BuzzLyseyear_107

BuzzLyseyear_108
(glycosyltransferase)

BuzzLyseyear_109

BuzzLyseyear_110
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKM347889
Refseq NumberNC_026593
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.5x10^10
Pitt Freezer Box# 3
Pitt Freezer Box Grid# D7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload