The Actinobacteriophage Database | Phage MisterCuddles

Mycobacterium phage MisterCuddles

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Detailed Information for Phage MisterCuddles
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Evan Witt
Year Found	2012
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	37°C
GPS Coordinates	38.644787 N, 90.311842 W Map
Discovery Notes	Based on ClaI digest, genome size of approximately 56 kb
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Oct 10, 2013
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	286
Sanger Finishing Reactions	4
Genome length (bp)	57754
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	University of South Florida
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	103
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View MisterCuddles_1 (terminase small subunit) MisterCuddles_2 (terminase large subunit) MisterCuddles_3 (portal protein) MisterCuddles_4 (capsid maturation protease) MisterCuddles_5 (scaffolding protein) MisterCuddles_6 (major capsid protein) MisterCuddles_7 (head-to-tail adaptor) MisterCuddles_8 (head-to-tail stopper) MisterCuddles_9 MisterCuddles_10 (tail terminator) MisterCuddles_11 (major tail protein) MisterCuddles_12 (tail assembly chaperone) MisterCuddles_13 (tail assembly chaperone) MisterCuddles_14 (tape measure protein) MisterCuddles_15 (minor tail protein) MisterCuddles_16 (minor tail protein) MisterCuddles_17 (minor tail protein) MisterCuddles_18 (minor tail protein) MisterCuddles_19 (D-ala-D-ala carboxypeptidase) MisterCuddles_20 MisterCuddles_21 MisterCuddles_22 MisterCuddles_23 MisterCuddles_24 MisterCuddles_25 (helix-turn-helix DNA binding domain protein) MisterCuddles_26 MisterCuddles_27 MisterCuddles_28 MisterCuddles_29 MisterCuddles_30 MisterCuddles_31 (lysin A) MisterCuddles_32 (HNH endonuclease) MisterCuddles_33 (lysin B) MisterCuddles_34 (holin) MisterCuddles_35 MisterCuddles_36 MisterCuddles_37 (DnaQ-like DNA polymerase III subunit) MisterCuddles_38 MisterCuddles_39 MisterCuddles_40 MisterCuddles_41 MisterCuddles_42 MisterCuddles_43 (tyrosine integrase) MisterCuddles_44 MisterCuddles_45 (immunity repressor) MisterCuddles_46 (helix-turn-helix DNA binding protein) MisterCuddles_47 (helix-turn-helix DNA binding domain protein) MisterCuddles_48 MisterCuddles_49 MisterCuddles_50 (excise) MisterCuddles_51 MisterCuddles_52 MisterCuddles_53 MisterCuddles_54 MisterCuddles_55 MisterCuddles_56 MisterCuddles_57 MisterCuddles_58 (helix-turn-helix DNA binding domain protein) MisterCuddles_59 MisterCuddles_60 MisterCuddles_61 MisterCuddles_62 MisterCuddles_63 (HNH endonuclease) MisterCuddles_64 (DNA helicase) MisterCuddles_65 MisterCuddles_66 MisterCuddles_67 MisterCuddles_68 (PAPS reductase-like domain protein) MisterCuddles_69 MisterCuddles_70 MisterCuddles_71 MisterCuddles_72 MisterCuddles_73 MisterCuddles_74 MisterCuddles_75 MisterCuddles_76 MisterCuddles_77 MisterCuddles_78 MisterCuddles_79 MisterCuddles_80 MisterCuddles_81 MisterCuddles_82 MisterCuddles_83 MisterCuddles_84 MisterCuddles_85 MisterCuddles_86 MisterCuddles_87 MisterCuddles_88 MisterCuddles_89 MisterCuddles_90 MisterCuddles_91 MisterCuddles_92 MisterCuddles_93 MisterCuddles_94 MisterCuddles_95 — tRNA (tRNA-Trp(cca)) MisterCuddles_96 MisterCuddles_97 MisterCuddles_98 MisterCuddles_99 MisterCuddles_100 MisterCuddles_101 (glycosyltransferase) MisterCuddles_102 MisterCuddles_103 (glycosyltransferase) MisterCuddles_104 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK359315
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	42
Pitt Freezer Box Grid#	22
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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