The Actinobacteriophage Database

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Detailed Information for Phage Rialto
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	unknown
Year Found	2018
Location Found	Butte, MT United States
Finding Institution	Montana Technological University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	4°C
GPS Coordinates	Unavailable
Discovery Notes	Emily Garden found the phage she named "Rialto" in plant bed soil from her backyard in Butte, Montana.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 5, 2023
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	3165
Genome length (bp)	57936
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CCGATGGCAT
GC Content	61.2%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	Montana Technological University
Annotation Status	In GenBank
Plaque Notes	The phage Rialto forms small consistent plaques. Currently, the PCR indicates that the phage Rialto is in the F cluster
Morphotype	Siphoviridae
Number of Genes	108
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Rialto_1 (HNH endonuclease) Rialto_2 (terminase small subunit) Rialto_3 (terminase large subunit) Rialto_4 (portal protein) Rialto_5 (capsid maturation protease) Rialto_6 (scaffolding protein) Rialto_7 (major capsid protein) Rialto_8 (head-to-tail adaptor) Rialto_9 (head-to-tail stopper) Rialto_10 Rialto_11 (tail terminator) Rialto_12 (major tail protein) Rialto_13 (tail assembly chaperone) Rialto_14 (tail assembly chaperone) Rialto_15 (HNH endonuclease) Rialto_16 (tape measure protein) Rialto_17 (minor tail protein) Rialto_18 (minor tail protein) Rialto_19 Rialto_20 (minor tail protein) Rialto_21 (minor tail protein) Rialto_22 (minor tail protein) Rialto_23 Rialto_24 Rialto_25 Rialto_26 Rialto_27 (helix-turn-helix DNA binding domain protein) Rialto_28 Rialto_29 Rialto_30 Rialto_31 (transposase) Rialto_32 Rialto_33 Rialto_34 (lysin A) Rialto_35 (lysin B) Rialto_36 (holin) Rialto_37 Rialto_38 Rialto_39 (DnaQ-like DNA polymerase III subunit) Rialto_40 Rialto_41 Rialto_42 Rialto_43 (hetero-immunity repressor) Rialto_44 Rialto_45 Rialto_46 (tyrosine integrase) Rialto_47 Rialto_48 Rialto_49 (immunity repressor) Rialto_50 (Cro protein) Rialto_51 (antirepressor) Rialto_52 Rialto_53 Rialto_54 Rialto_55 (excise) Rialto_56 Rialto_57 Rialto_58 Rialto_59 Rialto_60 Rialto_61 Rialto_62 Rialto_63 (WhiB family transcription factor) Rialto_64 (helix-turn-helix DNA binding domain protein) Rialto_65 (WhiB family transcription factor) Rialto_66 (helix-turn-helix DNA binding domain protein) Rialto_67 Rialto_68 Rialto_69 (SSB protein) Rialto_70 (HNH endonuclease) Rialto_71 (DNA methyltransferase) Rialto_72 Rialto_73 Rialto_74 Rialto_75 Rialto_76 (DNA methyltransferase) Rialto_77 Rialto_78 Rialto_79 Rialto_80 Rialto_81 (RepA-like replication initiator) Rialto_82 Rialto_83 Rialto_84 Rialto_85 Rialto_86 Rialto_87 (MPME1 protein) Rialto_88 Rialto_89 Rialto_90 Rialto_91 Rialto_92 Rialto_93 Rialto_94 Rialto_95 Rialto_96 Rialto_97 Rialto_98 Rialto_99 (G-I-Y Y-I-G endonuclease) Rialto_100 Rialto_101 Rialto_102 Rialto_103 Rialto_104 Rialto_105 Rialto_106 (glycosyltransferase) Rialto_107 Rialto_108 (glycosyltransferase)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OR475257
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	152
Pitt Freezer Box Grid#	F5
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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