| Detailed Information for Phage SuperGrey |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jacqueline Simeon and Aesha Newton-Ashley |
| Year Found | 2013 |
| Location Found | Nyack , NY USA |
| Finding Institution | Alliance University (formerly Nyack College) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.07851 N, 73.94125 W Map |
| Discovery Notes | Soil was obtained behind the North Campus building on the Nyack College campus, near a tree. It was 83 degrees Fahrenheit and sunny out that day. |
| Naming Notes | We decided to merge our name ideas of Christian Grey and Super Star. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2013 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3948 |
| Genome length (bp) | 59346 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Phage forms clear plaques which are approximately 3mm in 24 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX808131 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.8 x 10^11 |
| Date of SEA Lysate Titering |
Oct 24, 2013 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |