| Detailed Information for Phage Chevrolet |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Daphne Ngape |
| Year Found | 2023 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.4462 N, 79.95712 W Map |
| Discovery Notes | Found near the Chevron Science Center. Soil collection was under shrubs in an area recently landscaped. Fertilizer was visible in the soil. Shrubs were next to a sidewalk and road. Soil collected was found no more than 3 inches underground at 18.3 degrees Celsius. Dry hard soil had to be uncovered to get to softer soil for the sample collected. |
| Naming Notes | Chevrolet sounds similar to Chevron |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 19777 |
| Genome length (bp) | 58570 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184830 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
1.33 x 10^10 |
| Available Files |
| GenBank File for Phamerator | Download |