| Detailed Information for Phage DosHalletts |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Benjamin Hallett and Ethan Hallett |
| Year Found | 2023 |
| Location Found | Ellsworth, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.555 N, 68.4134 W Map |
| Discovery Notes | DosHalletts seems to be a highly aggressive phage. We expanded our titer from 10^5 to 10^12 after only one sample enrichment. |
| Naming Notes | My lab partner and I came up with this name because by coincidence, both of our last names happen to be Hallett. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 928 |
| Genome length (bp) | 57562 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | The Phage DosHalletts will formed plaques 36% of the time when given the option when testing with a virus. |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | The Phage DosHalletts will form a plaque about 2-3 millimeters in diameter. We have only ever observed clear plaques (Lytic), and have yet to observe a turbid (Temperate) plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978828 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
