| Detailed Information for Phage DotProduct |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Andryus Planutis/Mahathee Chetapalli |
| Year Found | 2009 |
| Location Found | Irvine, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.64035 N, 117.83217 W Map |
| Discovery Notes | Located UC Irvine Community Gardens. It was a moist compost, well, tended, in bin with ventilation holes. Soil depth was 10cm at 86 degrees. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 17, 2011 |
| Sequencing Facility | Joint Genomics Institute |
| Genome length (bp) | 55363 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 4mm diameter/clear dot plaques after 24 hours of incubation, turned into bulls-eye plaques after 24 more hours of incubation ( total incubation 48 hours). at 37 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JN859129 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Virtual Digest Picture | Download |
| EM Picture | Download |
