| Detailed Information for Phage Estave1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Paige Estave |
| Year Found | 2012 |
| Location Found | Kenner, LA USA |
| Finding Institution | Xavier University of Louisiana |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.999444 N, 90.233889 W Map |
| Discovery Notes | The soil sample was collected at 2400 Kentucky Ave. Kenner, LA 70062. The sample specifically came from a garden containing a plant for egg plants. The soil in the garden was composed of Miracle Gro for flowers and vegetables. There was barely any decaying matter in the area. The soil itself contained very little moisture and was light brown. |
| Naming Notes | The name of the phage is the founder's last name plus a number associated with its discovery (being the first one found). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 340 |
| Sanger Finishing Reactions | 10 |
| Genome length (bp) | 60727 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques of Estave1 are small (~0.5mm-1.25 mm), completely clear and circular. There are two distinguishable sizes: small and even smaller. |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM279937 |
| Refseq Number | NC_026585 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
41 |
| Pitt Freezer Box Grid# |
66 |