The Actinobacteriophage Database

Mycobacterium phage KingMidas

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Detailed Information for Phage KingMidas
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Zachary Jordan
Year Found	2016
Location Found	Providence, RI USA
Finding Institution	Brown University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	23°C
GPS Coordinates	40.27018 N, 97.126 W Map
Discovery Notes	This soil was collected from underneath a sprinkler head in Keeney Quad, at Brown University. It consisted mostly of decomposing leaf and plant matter, and was damp. Collected at night, when the soil was wettest. Coordinates recorded above are likely wrong.Not consistent with the discovery notes, but put collection site in Nebraska. Scottish, a related virus nearly identical to Kingmidas was found in Keeney Quad at 41.824167 N, 71.403333 W.
Naming Notes	King Midas is a figure in Greek Mythology best known for his ability to turn objects or people to gold by touching them. As a result, he killed everything living that he touched, as does this phage with bacteria.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 3, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1672
Genome length (bp)	57386
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) Akhila (F1) Alexphander (F1) Aloeri (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Brookers (F1) Brushbloom (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Cheesepuff (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Corium (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) DoRead (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) EmiMonkey (F1) Emma (F1) Emmaloid (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) Grimmer (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jant (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingJulian (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Mwanjiwa (F1) NewHope4 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Peridot (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) PhlipPhlop (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) Polo2Bam (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Slim (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) Tchotchke (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Topiatin (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) Violac (F1) Vivum (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) SilverChicken (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6) MulchSalad (F7)
Annotating Institution	College of Charleston
Annotation Status	In GenBank
Plaque Notes	Plaques are clear, indicating a lytic phage. Diameter range: 1.46 – 3.24 mm (mean = 2.41 mm plus or minus 0.1 mm SE). Fairly consistent size and circular shape, with randomized distribution throughout the agar.
Morphotype	Siphoviridae
Number of Genes	105
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View KingMidas_1 (terminase small subunit) KingMidas_2 (terminase large subunit) KingMidas_3 (portal protein) KingMidas_4 (capsid maturation protease) KingMidas_5 (scaffolding protein) KingMidas_6 (major capsid protein) KingMidas_7 (head-to-tail adaptor) KingMidas_8 (head-to-tail stopper) KingMidas_9 KingMidas_10 (tail terminator) KingMidas_11 (major tail protein) KingMidas_12 (tail assembly chaperone) KingMidas_13 (tail assembly chaperone) KingMidas_14 (tape measure protein) KingMidas_15 (transposase) KingMidas_16 (minor tail protein) KingMidas_17 (minor tail protein) KingMidas_18 KingMidas_19 (minor tail protein) KingMidas_20 (minor tail protein) KingMidas_21 KingMidas_22 KingMidas_23 KingMidas_24 KingMidas_25 KingMidas_26 (helix-turn-helix DNA-binding domain protein) KingMidas_27 KingMidas_28 KingMidas_29 KingMidas_30 KingMidas_31 (lysin A) KingMidas_32 (HNH endonuclease) KingMidas_33 (lysin B) KingMidas_34 KingMidas_35 KingMidas_36 KingMidas_37 (DnaQ-like DNA polymerase III subunit) KingMidas_38 KingMidas_39 KingMidas_40 KingMidas_41 KingMidas_42 (excise) KingMidas_43 KingMidas_44 (tyrosine integrase) KingMidas_45 KingMidas_46 KingMidas_47 (immunity repressor) KingMidas_48 (Cro protein) KingMidas_49 KingMidas_50 (helix-turn-helix DNA-binding domain protein) KingMidas_51 KingMidas_52 KingMidas_53 KingMidas_54 KingMidas_55 KingMidas_56 (WhiB family transcription factor) KingMidas_57 (helix-turn-helix DNA-binding domain protein) KingMidas_58 (WhiB family transcription factor) KingMidas_59 (helix-turn-helix DNA-binding domain protein) KingMidas_60 KingMidas_61 KingMidas_62 (Ku-like dsDNA break-binding protein) KingMidas_63 (RecA-like DNA recombinase) KingMidas_64 KingMidas_65 (HNH endonuclease) KingMidas_66 KingMidas_67 KingMidas_68 (DnaQ-like DNA polymerase III subunit) KingMidas_69 (DNA methylase) KingMidas_70 (DNA methylase) KingMidas_71 KingMidas_72 KingMidas_73 (DNA methylase) KingMidas_74 KingMidas_75 (helix-turn-helix DNA-binding domain protein) KingMidas_76 KingMidas_77 KingMidas_78 KingMidas_79 KingMidas_80 KingMidas_81 KingMidas_82 KingMidas_83 KingMidas_84 KingMidas_85 KingMidas_86 KingMidas_87 KingMidas_88 KingMidas_89 KingMidas_90 KingMidas_91 KingMidas_92 KingMidas_93 KingMidas_94 (helix-turn-helix DNA-binding domain protein) KingMidas_95 KingMidas_96 KingMidas_97 KingMidas_98 KingMidas_99 KingMidas_100 KingMidas_101 (glycosyltransferase) KingMidas_102 KingMidas_103 KingMidas_104 (glycosyltransferase) KingMidas_105 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN204502
Refseq Number	None yet
Published in Paper	Byrum et al 2020, Microbiology Resource Announcements: Complete Genome Sequence of the Cluster F1 Mycobacteriophage KingMidas
Archiving Info
Archiving status	Not in Pitt Archives
SEA Lysate Titer	2.21 x 10^11
Date of SEA Lysate Titering	Oct 12, 2016
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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