| Detailed Information for Phage Krakatau |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Agustin Borjon |
| Year Found | 2008 |
| Location Found | Santa Cruz, CA USA |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.999383 N, 122.05305 W Map |
| Discovery Notes | Taken from a compost Heap, depth five inches, 77°F, at the UCSC Merrill College- Merrill Gardens. Several compost heaps lined the road of the Merrill Gardens. The one I chose to collect from was the second from the left. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2012 |
| Genome length (bp) | 53058 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCGC |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Approximately 1.5mm radius. Clear with turbid halo surrounded by clear ring
24 hour incubation period in 37oC conditions
|
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH590598 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008ucscKrakatau |
| SEA Lysate Titer |
2.8X10^10 |
| Pitt Freezer Box# |
6 |
| Pitt Freezer Box Grid# |
73 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
