| Detailed Information for Phage Misha28 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Madeline Manfra-Levitt |
| Year Found | 2016 |
| Location Found | Charlton, MA USA |
| Finding Institution | Worcester Polytechnic Institute |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.120573 N, 71.98548 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 22, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1969 |
| Genome length (bp) | 57455 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGAAGGCAT |
| GC Content | 62.9% |
| Sequencing Notes | 1 nucleotide different than TootsiePop, isolated from same sample |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Worcester Polytechnic Institute |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH020242 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.1X10^10 |
| Date of SEA Lysate Titering |
Dec 14, 2016 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |