The Actinobacteriophage Database

Mycobacterium phage Spoonbill

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Detailed Information for Phage Spoonbill
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Kevin Ko and Valerie Bostrom
Year Found	2010
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.635526 N, 90.287562 W Map
Discovery Notes	Taken from the Flight Cage at the St. Louis Zoo on Sept. 5, 2010 Mixture of soil and "bird poop," bird possibly Roseate Spoonbill (Platalea ajaja) Surface of damp soil, under a cypress tree, near a pond
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 12, 2012
Genome length (bp)	55735
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.3%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) Akhila (F1) Alexphander (F1) Aloeri (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Brookers (F1) Brushbloom (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Cheesepuff (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Corium (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) DoRead (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) EmiMonkey (F1) Emma (F1) Emmaloid (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) Grimmer (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jant (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingJulian (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Medindi (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Mwanjiwa (F1) NewHope4 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Peridot (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) PhlipPhlop (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) Polo2Bam (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Slim (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) Tchotchke (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Topiatin (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) Violac (F1) Vivum (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) SilverChicken (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6) MulchSalad (F7)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Size: ~2 mm
Morphotype	Siphoviridae
Number of Genes	103
Number of tRNAs	0
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Spoonbill_1 (terminase small subunit) Spoonbill_2 (terminase large subunit) Spoonbill_3 (portal protein) Spoonbill_4 (capsid maturation protease) Spoonbill_5 (scaffolding protein) Spoonbill_6 (major capsid protein) Spoonbill_7 (head-to-tail adaptor) Spoonbill_8 (head-to-tail stopper) Spoonbill_9 Spoonbill_10 (tail terminator) Spoonbill_11 (major tail protein) Spoonbill_12 (tail assembly chaperone) Spoonbill_13 (tail assembly chaperone) Spoonbill_14 (tape measure protein) Spoonbill_15 (minor tail protein) Spoonbill_16 (minor tail protein) Spoonbill_17 (minor tail protein) Spoonbill_18 (minor tail protein) Spoonbill_19 (minor tail protein) Spoonbill_20 (minor tail protein) Spoonbill_21 (minor tail protein) Spoonbill_22 Spoonbill_23 (minor tail protein) Spoonbill_24 (minor tail protein) Spoonbill_25 (helix-turn-helix DNA-binding domain protein) Spoonbill_26 Spoonbill_27 Spoonbill_28 Spoonbill_29 Spoonbill_30 (lysin A) Spoonbill_31 (lysin B) Spoonbill_32 (holin) Spoonbill_33 Spoonbill_34 Spoonbill_35 (DnaQ-like DNA polymerase III subunit) Spoonbill_36 Spoonbill_37 Spoonbill_38 Spoonbill_39 Spoonbill_40 (excise) Spoonbill_41 Spoonbill_42 (tyrosine integrase) Spoonbill_43 Spoonbill_44 Spoonbill_45 Spoonbill_46 (helix-turn-helix DNA-binding domain protein) Spoonbill_47 Spoonbill_48 Spoonbill_49 Spoonbill_50 Spoonbill_51 Spoonbill_52 Spoonbill_53 Spoonbill_54 (WhiB family transcription factor) Spoonbill_55 Spoonbill_56 Spoonbill_57 (helix-turn-helix DNA-binding domain protein) Spoonbill_58 Spoonbill_59 Spoonbill_60 Spoonbill_61 (RecA-like DNA recombinase) Spoonbill_62 Spoonbill_63 (HNH endonuclease) Spoonbill_64 — tmRNA Spoonbill_65 (DNA methylase) Spoonbill_66 Spoonbill_67 (DNA methylase) Spoonbill_68 Spoonbill_69 Spoonbill_70 Spoonbill_71 (GIY-YIG endonuclease) Spoonbill_72 Spoonbill_73 Spoonbill_74 Spoonbill_75 Spoonbill_76 Spoonbill_77 Spoonbill_78 Spoonbill_79 (MPME1 protein) Spoonbill_80 Spoonbill_81 Spoonbill_82 Spoonbill_83 Spoonbill_84 Spoonbill_85 Spoonbill_86 Spoonbill_87 Spoonbill_88 Spoonbill_89 Spoonbill_90 Spoonbill_91 (GIY-YIG endonuclease) Spoonbill_92 Spoonbill_93 Spoonbill_94 Spoonbill_95 Spoonbill_96 Spoonbill_97 Spoonbill_98 Spoonbill_99 Spoonbill_100 Spoonbill_101 (glycosyltransferase) Spoonbill_102 Spoonbill_103 (glycosyltransferase) Spoonbill_104 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF190168
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	WUSL
SEA Lysate Titer	1.68e12
Date of SEA Lysate Titering	Oct 7, 2010
Pitt Freezer Box#	20
Pitt Freezer Box Grid#	32
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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