| Detailed Information for Phage Wachhund |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Dimond Shelton |
| Year Found | 2016 |
| Location Found | Cromwell, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.351064 N, 86.77467 W Map |
| Discovery Notes | I gathered this sample from my dog's pen at 7:16AM on September 12, 2016. I dug down approximately 2.5 inches when gathering my sample. There was minimal direct sunlight in this area, and the ground temperature was 12.2°C. The soil was moist. |
| Naming Notes | Since I gathered this sample from my dog's pen, I wanted to name my phage something that reminded me of where I found my sample. My dog is a purebred German Shepherd and was trained to protect our property. Wachhund is German for "guard dog." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2423 |
| Genome length (bp) | 54513 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are moderately small and extremely clear on plates, but exhibit faint halos around the clear plaque center. The clear plaque in the center is 1 millimeter in diameter with a turbid halo 2 millimeters in radius surrounding. These halos were only visible out to the 10^-8 dilution. At lower dilutions, the phage appeared to be lytic. As the space between individual plaques increased at higher dilutions, "bull's eyes" could be seen around the clear plaques. Plates were, typically, incubated at 30°C for 48 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF668287 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUwachhundDS |
| SEA Lysate Titer |
2.7 x 10^10 pfu/mL |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
