| Detailed Information for Phage ActinUp |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | N/A |
| Found By | Yorich Poole, Lucas Galassi |
| Year Found | 2016 |
| Location Found | Greensburg, PA USA |
| Finding Institution | Seton Hill University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 33°C |
| GPS Coordinates | 40.310755 N, 79.559596 W Map |
| Discovery Notes | I discovered this phage in soil directly outside of Brownlee Residence Hall. |
| Naming Notes | Albeit this is not an Actinophage, the name brought a wide smile to my petite face. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 21, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1653 |
| Genome length (bp) | 59812 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CTCGTAGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Seton Hill University |
| Annotation Status | In GenBank |
| Plaque Notes | Large, round, turbid plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH051246 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.3 x 10^11 |
| Pitt Freezer Box# |
33 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| GenBank File for Phamerator | Download |