Mycobacterium phage Peel
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Detailed Information for Phage Peel
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJessica Guillaume
Year Found2014
Location FoundGrand Rapids, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates42.866038 N, 85.619162 W Map
Discovery NotesPeel was isolated through enrichment methods and purified at 32°C.
Naming NotesThis phage was found in a compost garden.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 8, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4523
Genome length (bp)59711
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesPeel is capable of growing at 42°C. Cells were extracted from mesas formed after 5 days of incubation, and were tested for superinfection by the same phage. Our results yielded the formation of many plaques, suggesting Peel is temperate and capable of producing stable lysogen.
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUniversity of the Ozarks
Annotation StatusIn GenBank
Plaque NotesClear centers surrounded by turbid rings. Approximately 2 mm in diameter. Turbid ring does not form until after 24 hours.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Peel_1

Peel_2

Peel_3

Peel_4

Peel_6

Peel_7

Peel_8
(terminase)

Peel_9
(portal protein)

Peel_10
(MuF-like minor capsid protein)

Peel_11

Peel_12
(scaffolding protein)

Peel_13
(major capsid protein)

Peel_14
(head-to-tail adaptor)

Peel_15
(head-to-tail stopper)

Peel_16

Peel_17
(tail terminator)

Peel_18
(major tail protein)

Peel_19
(tail assembly chaperone)

Peel_20
(tail assembly chaperone)

Peel_21
(tape measure protein)

Peel_22
(minor tail protein)

Peel_23
(minor tail protein)

Peel_24
(minor tail protein)

Peel_25
(minor tail protein)

Peel_26

Peel_27
(minor tail protein)

Peel_28
(minor tail protein)

Peel_29

Peel_30
(lysin A)

Peel_31
(lysin B)

Peel_32
(holin)

Peel_33

Peel_34

Peel_35
(MRE11 double-strand break endo/exonuclease)

Peel_36
(helix-turn-helix DNA binding protein)

Peel_37

Peel_38

Peel_39

Peel_40

Peel_41
(tyrosine integrase)

Peel_42

Peel_43
(immunity repressor)

Peel_44
(helix-turn-helix DNA binding domain)

Peel_45
(excise)

Peel_46

Peel_47

Peel_48

Peel_49

Peel_50
(WhiB family transcription factor)

Peel_51

Peel_52

Peel_53

Peel_54

Peel_55

Peel_56

Peel_57
(DnaQ-like (DNA polymerase III subunit))

Peel_58

Peel_59

Peel_60

Peel_61
(exonuclease)

Peel_62

Peel_63

Peel_64

Peel_65

Peel_66
(NrdH-like glutaredoxin)

Peel_67

Peel_68
(HNH endonuclease)

Peel_69
(DNA primase/helicase)

Peel_70

Peel_71
(RusA-like resolvase (endonuclease))

Peel_72

Peel_73

Peel_74

Peel_75

Peel_76
(SprT-like protease)

Peel_77

Peel_78

Peel_79

Peel_80

Peel_81

Peel_82

Peel_83

Peel_84

Peel_85
(rtcB-like RNA ligase)

Peel_86
(DNA binding protein)

Peel_87

Peel_88

Peel_89

Peel_90

Peel_91
(antitoxin in toxin/antitoxin system, HicB-like)

Peel_92
(toxin in toxin/antitoxin system, HicA-like)

Peel_93

Peel_94

Peel_95

Peel_96
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW862979
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.8e10
Pitt Freezer Box# 7
Pitt Freezer Box Grid# F12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload