Mycobacterium phage ActinUp
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Detailed Information for Phage ActinUp
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesN/A
Found ByYorich Poole, Lucas Galassi
Year Found2016
Location FoundGreensburg, PA USA
Finding InstitutionSeton Hill University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature33°C
GPS Coordinates40.310755 N, 79.559596 W Map
Discovery NotesI discovered this phage in soil directly outside of Brownlee Residence Hall.
Naming NotesAlbeit this is not an Actinophage, the name brought a wide smile to my petite face.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1653
Genome length (bp)59812
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionSeton Hill University
Annotation StatusIn GenBank
Plaque NotesLarge, round, turbid plaque.
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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ActinUp_1

ActinUp_2

ActinUp_3

ActinUp_4

ActinUp_5 — tRNA
(tRNA-trp (cca))

ActinUp_6

ActinUp_7

ActinUp_8
(terminase)

ActinUp_9
(portal protein)

ActinUp_10
(MuF-like minor capsid protein)

ActinUp_11

ActinUp_12
(scaffolding protein)

ActinUp_13
(major capsid protein)

ActinUp_14
(head-to-tail adaptor)

ActinUp_15
(head-to-tail stopper)

ActinUp_16

ActinUp_17
(tail terminator)

ActinUp_18

ActinUp_19

ActinUp_20

ActinUp_21
(tape measure protein)

ActinUp_22
(minor tail protein)

ActinUp_23
(minor tail protein)

ActinUp_24
(minor tail protein)

ActinUp_25
(minor tail protein)

ActinUp_26
(minor tail protein)

ActinUp_27
(minor tail protein)

ActinUp_28
(minor tail protein)

ActinUp_29

ActinUp_30
(lysin A)

ActinUp_31
(lysin B)

ActinUp_32
(holin)

ActinUp_33

ActinUp_34

ActinUp_35
(MRE11 double-strand break endo/exonuclease)

ActinUp_36

ActinUp_37

ActinUp_38

ActinUp_39

ActinUp_40

ActinUp_41
(tyrosine integrase)

ActinUp_42

ActinUp_43
(immunity repressor)

ActinUp_44
(helix-turn-helix DNA binding protein)

ActinUp_45
(excise)

ActinUp_46

ActinUp_47

ActinUp_48

ActinUp_49
(MerR-like helix-turn-helix DNA binding domain protein)

ActinUp_50
(WhiB family transcription factor)

ActinUp_51

ActinUp_52

ActinUp_53

ActinUp_54

ActinUp_55

ActinUp_56

ActinUp_57
(DnaE-like DNA polymerase III)

ActinUp_58

ActinUp_59

ActinUp_60

ActinUp_61
(Cas4 family endonuclease)

ActinUp_62

ActinUp_63

ActinUp_64

ActinUp_65

ActinUp_66
(NrdH-like glutaredoxin)

ActinUp_67

ActinUp_68
(HNH endonuclease)

ActinUp_69
(DNA primase/helicase)

ActinUp_70

ActinUp_71
(RusA-like resolvase)

ActinUp_72

ActinUp_73

ActinUp_74

ActinUp_75

ActinUp_76

ActinUp_77
(SprT-like protease)

ActinUp_78

ActinUp_79

ActinUp_80

ActinUp_81

ActinUp_82

ActinUp_83

ActinUp_84

ActinUp_85

ActinUp_86
(RtcB-like RNA ligase)

ActinUp_87

ActinUp_88

ActinUp_89

ActinUp_90

ActinUp_91

ActinUp_92
(HicB-like antitoxin)

ActinUp_93
(HicA-like toxin)

ActinUp_94

ActinUp_95

ActinUp_96

ActinUp_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH051246
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.3 x 10^11
Pitt Freezer Box# 33
Pitt Freezer Box Grid# E10
Available Files
GenBank File for PhameratorDownload