Detailed Information for Phage OkiRoe |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Xiao Xiao |
Year Found | 2013 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.44405 N, 79.955039 W Map |
Discovery Notes | On 09/11/13, a day after it rained, I collected OkiRoe from an area of slightly damp soil from a lawn of grass. |
Naming Notes | For sushi lovers, you will notice how mycobacteriophage plaques look deliciously similar to fish eggs/caviar. Thus, I gave my phage a Japanese name that means "big fish eggs" ("Oki" means "big" and "roe" means "fish eggs"). |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Nov 14, 2013 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 6692 |
Genome length (bp) | 62661 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CTCAGTGGCAT |
GC Content | 64.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K5 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | Phage is capable of lysing M. smegmatis. |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KJ567042 |
Refseq Number | NC_024366 |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
1 |
Pitt Freezer Box Grid# |
B8 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |