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Mycobacterium phage OkiRoe
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Detailed Information for Phage OkiRoe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByXiao Xiao
Year Found2013
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.44405 N, 79.955039 W Map
Discovery NotesOn 09/11/13, a day after it rained, I collected OkiRoe from an area of slightly damp soil from a lawn of grass.
Naming NotesFor sushi lovers, you will notice how mycobacteriophage plaques look deliciously similar to fish eggs/caviar. Thus, I gave my phage a Japanese name that means "big fish eggs" ("Oki" means "big" and "roe" means "fish eggs").
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 14, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage6692
Genome length (bp)62661
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content64.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Lysogeny NotesPhage is capable of lysing M. smegmatis.
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

OkiRoe_1

OkiRoe_2

OkiRoe_3

OkiRoe_5

OkiRoe_6
(terminase)

OkiRoe_7
(portal protein)

OkiRoe_8
(MuF-like minor capsid protein)

OkiRoe_9

OkiRoe_10
(scaffolding protein)

OkiRoe_11
(major capsid protein)

OkiRoe_12
(head-to-tail adaptor)

OkiRoe_13
(head-to-tail stopper)

OkiRoe_14

OkiRoe_15
(tail terminator)

OkiRoe_16
(major tail protein)

OkiRoe_17
(tail assembly chaperone)

OkiRoe_18
(tail assembly chaperone)

OkiRoe_19
(tape measure protein)

OkiRoe_20
(minor tail protein)

OkiRoe_21
(minor tail protein)

OkiRoe_22
(minor tail protein)

OkiRoe_23
(minor tail protein)

OkiRoe_24

OkiRoe_25
(minor tail protein)

OkiRoe_26

OkiRoe_27
(minor tail protein)

OkiRoe_28

OkiRoe_29
(lysin A)

OkiRoe_30
(lysin B)

OkiRoe_31
(holin)

OkiRoe_32

OkiRoe_33

OkiRoe_34

OkiRoe_35

OkiRoe_36

OkiRoe_37

OkiRoe_38
(serine integrase)

OkiRoe_39

OkiRoe_40

OkiRoe_41
(immunity repressor)

OkiRoe_42

OkiRoe_43

OkiRoe_44

OkiRoe_45

OkiRoe_46

OkiRoe_47
(DNA polymerase III sliding clamp)

OkiRoe_48

OkiRoe_49

OkiRoe_50

OkiRoe_51

OkiRoe_52

OkiRoe_53

OkiRoe_54
(ssDNA binding protein)

OkiRoe_55

OkiRoe_56

OkiRoe_57

OkiRoe_58

OkiRoe_59

OkiRoe_60

OkiRoe_61
(NrdH-like glutaredoxin)

OkiRoe_62
(HNH endonuclease)

OkiRoe_63
(HNH endonuclease)

OkiRoe_64
(DNA primase/polymerase)

OkiRoe_65
(DNA helicase)

OkiRoe_66
(holliday junction resolvase)

OkiRoe_67

OkiRoe_68

OkiRoe_69

OkiRoe_70

OkiRoe_71

OkiRoe_72

OkiRoe_73

OkiRoe_74

OkiRoe_75

OkiRoe_76

OkiRoe_77

OkiRoe_78

OkiRoe_79
(RtcB-like RNA ligase)

OkiRoe_80

OkiRoe_81

OkiRoe_82

OkiRoe_83

OkiRoe_84

OkiRoe_85

OkiRoe_86

OkiRoe_87

OkiRoe_88

OkiRoe_89

OkiRoe_90

OkiRoe_91

OkiRoe_92

OkiRoe_93

OkiRoe_94

OkiRoe_95

OkiRoe_96

OkiRoe_97
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ567042
Refseq NumberNC_024366
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload