The Actinobacteriophage Database

Mycobacterium phage Deby

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Detailed Information for Phage Deby
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Yiwei Sun
Year Found	2017
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	34.039394 N, 118.4423 W Map
Discovery Notes	Phage Deby was isolated by Yiwei Sun on 04/8/2017 from moist soil collected in an urban area, directly beneath metal pipes, next to building and pedestrian walk. Phage Deby was isolated by enrichment plating at 30C. Characterization was performed by Team (KR)^2 (Keeyon Dabirian, Rachel Desai, Kyerra Jones, Ryan Toan Ngo) and Phantastic Phour (Diane Park, Erin Sakaji, Yiwei Sun, Boon Xin Tan). Name of Individual who collected sample: Yiwei Sun Midi-Lysate (Archive) Titer: 7x1010 PFU/ml Date of Midi-Lysate Titer: 05/16/2017 AL Quarter and Lab section (ie. S15-1A): S17-1B Instructor: Dr. Jordan Moberg Parker Teaching Assistant: Andrew Lund Undergraduate Assistant: Sam Wu
Naming Notes	This phage was named "Deby" because it is an acronym of the Phantastic Phour team members' names.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 29, 2017
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Genome length (bp)	60463
Character of genome ends	Unknown
Overhang Length	11 bases
Overhang Sequence	CTCGTAGGCAT
GC Content	66.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) Bern (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) CallaLilly (K1) Capricorn (K1) CaseJules (K1) Chavito (K1) CheetoDust (K1) Chris (K1) Clipper (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) DartGoblin (K1) Dartin (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MacKat (K1) MarkPhew (K1) McMater (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pisa4 (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Richo (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) Teejan (K1) TingHuaYa (K1) TiniBug (K1) Tiri (K1) Topper (K1) Torres (K1) TreyKay (K1) Trice (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Doughnut (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) Lea83 (K3) MacnCheese (K3) Neighly (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Bobquesha (K4) Chancellor (K4) Cheetobro (K4) DanSyl44 (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Kraw (K4) Lebo14 (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) YasnayaPolyana (K4) Agent47 (K5) AlleyCat (K5) BananenDer11 (K5) Collard (K5) Dadosky (K5) DoubleChamp (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) ViviJ (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Applecrisp (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) November (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Plaque Notes	halo (clear center with turbid edges) Average size (and size range) diameter in mm: 3.82± 0.69mm ; 3mm-4mm
Morphotype	Siphoviridae
Number of Genes	95
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Deby_1 Deby_2 Deby_3 Deby_4 Deby_5 — tRNA (tRNA-Trp(cca)) Deby_6 Deby_7 Deby_8 (terminase) Deby_9 (portal protein) Deby_10 (MuF-like minor capsid protein) Deby_11 Deby_12 (scaffolding protein) Deby_13 (major capsid protein) Deby_14 (head-to-tail adaptor) Deby_15 (head-to-tail stopper) Deby_16 Deby_17 (tail terminator) Deby_18 Deby_19 Deby_20 Deby_21 (tape measure protein) Deby_22 (minor tail protein) Deby_23 (minor tail protein) Deby_24 (minor tail protein) Deby_25 (minor tail protein) Deby_26 (minor tail protein) Deby_27 (minor tail protein) Deby_28 (minor tail protein) Deby_29 Deby_30 (lysin A) Deby_31 (lysin B) Deby_32 (holin) Deby_33 Deby_34 Deby_35 (MRE11 double-strand break endo/exonuclease) Deby_36 Deby_37 Deby_38 Deby_39 Deby_40 Deby_41 (tyrosine integrase) Deby_42 Deby_43 (immunity repressor) Deby_44 (helix-turn-helix DNA binding protein) Deby_45 (excise) Deby_46 Deby_47 Deby_48 Deby_49 (MerR-like helix-turn-helix DNA binding domain protein) Deby_50 (WhiB family transcription factor) Deby_51 Deby_52 Deby_53 Deby_54 Deby_55 Deby_56 Deby_57 (DnaE-like DNA polymerase III) Deby_58 Deby_59 Deby_60 Deby_61 (Cas4 family endonuclease) Deby_62 Deby_63 Deby_64 Deby_65 Deby_66 Deby_67 (NrdH-like glutaredoxin) Deby_68 (HNH endonuclease) Deby_69 (DNA primase/helicase) Deby_70 (RusA-like resolvase) Deby_71 Deby_72 Deby_73 Deby_74 Deby_75 Deby_76 (SprT-like protease) Deby_77 Deby_78 Deby_79 Deby_80 Deby_81 Deby_82 Deby_83 Deby_84 Deby_85 (RtcB-like RNA ligase) Deby_86 Deby_87 Deby_88 Deby_89 Deby_90 Deby_91 (HicB-like antitoxin) Deby_92 (HicA-like toxin) Deby_93 Deby_94 Deby_95 Deby_96 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MG962364
Refseq Number	None yet
Published in Paper	Gaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley.
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	45
Pitt Freezer Box Grid#	D9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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