Mycobacterium phage Sunflower1121
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Detailed Information for Phage Sunflower1121
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLee Monroe
Year Found2021
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986898 N, 86.45418 W Map
Discovery NotesWhen this phage was discovered it was around 74 degrees Fahrenheit. The sky was overcast and there was a light drizzle of rain. It was about 7pm CT.
Naming NotesThis name was produced from some very sentimental things in my life. This was a nickname and a very important date that I combined to name my phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage669
Genome length (bp)60213
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCGGGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK6
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesThis phage produces very turbid plaques with smooth edges. It typically has a diameter around 0.5cm (5mm) across.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Sunflower1121_1

Sunflower1121_2

Sunflower1121_3

Sunflower1121_4

Sunflower1121_5

Sunflower1121_6
(terminase)

Sunflower1121_7
(portal protein)

Sunflower1121_8
(capsid maturation protease)

Sunflower1121_9

Sunflower1121_10
(scaffolding protein)

Sunflower1121_11
(major capsid protein)

Sunflower1121_12
(head-to-tail adaptor)

Sunflower1121_13
(head-to-tail stopper)

Sunflower1121_14

Sunflower1121_15
(tail terminator)

Sunflower1121_16
(major tail protein)

Sunflower1121_17
(tail assembly chaperone)

Sunflower1121_18
(tail assembly chaperone)

Sunflower1121_19
(tape measure protein)

Sunflower1121_20
(minor tail protein)

Sunflower1121_21
(minor tail protein)

Sunflower1121_22

Sunflower1121_23

Sunflower1121_24
(minor tail protein)

Sunflower1121_25
(minor tail protein)

Sunflower1121_26

Sunflower1121_27

Sunflower1121_28

Sunflower1121_29
(lysin A)

Sunflower1121_30
(lysin B)

Sunflower1121_31
(holin)

Sunflower1121_32

Sunflower1121_33

Sunflower1121_34
(MRE11 double-strand break endo/exonuclease)

Sunflower1121_35

Sunflower1121_36

Sunflower1121_37
(helix-turn-helix DNA binding domain)

Sunflower1121_38

Sunflower1121_39

Sunflower1121_40

Sunflower1121_41

Sunflower1121_42

Sunflower1121_43

Sunflower1121_44

Sunflower1121_45

Sunflower1121_46
(tyrosine integrase)

Sunflower1121_47

Sunflower1121_48

Sunflower1121_49
(immunity repressor)

Sunflower1121_50
(Cro (control of repressor)

Sunflower1121_51
(excise)

Sunflower1121_52

Sunflower1121_53

Sunflower1121_54

Sunflower1121_55

Sunflower1121_56

Sunflower1121_57
(WhiB family transcription factor)

Sunflower1121_58

Sunflower1121_59

Sunflower1121_60

Sunflower1121_61

Sunflower1121_62

Sunflower1121_63
(exonuclease)

Sunflower1121_64

Sunflower1121_65
(Cas4 family exonuclease)

Sunflower1121_66

Sunflower1121_67

Sunflower1121_68

Sunflower1121_69

Sunflower1121_70

Sunflower1121_71

Sunflower1121_72
(NrdH-like glutaredoxin)

Sunflower1121_73
(HNH endonuclease)

Sunflower1121_74
(DNA primase/helicase)

Sunflower1121_75

Sunflower1121_76
(RusA-like resolvase (endonuclease))

Sunflower1121_77

Sunflower1121_78
(helix-turn-helix DNA binding domain, MerR-like)

Sunflower1121_79

Sunflower1121_80

Sunflower1121_81

Sunflower1121_82
(SprT-like protease)

Sunflower1121_83

Sunflower1121_84

Sunflower1121_85

Sunflower1121_86

Sunflower1121_87
(helix-turn-helix DNA binding domain, MerR-like)

Sunflower1121_88

Sunflower1121_89

Sunflower1121_90
(rtcB-like RNA ligase)

Sunflower1121_91

Sunflower1121_92

Sunflower1121_93

Sunflower1121_94

Sunflower1121_95

Sunflower1121_96

Sunflower1121_97

Sunflower1121_98

Sunflower1121_99

Sunflower1121_100
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456351
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.25 x 10^10 pfu/ml
Pitt Freezer Box# 138
Pitt Freezer Box Grid# A3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload