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Mycobacterium phage Boiiii
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Detailed Information for Phage Boiiii
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKyle Brendan Cushman
Year Found2014
Location FoundMidland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.788333 N, 86.105278 W Map
Discovery NotesThe soil was collected on 8/23/14, the air temperature was 28.3 degrees Celcius, the soil came from right on the surface, the soil was dry, and the it was collected in an urban environment (on Hope College's campus).
Naming NotesThe name Boiiii came from an inside joke with one of my classmates and I.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 4, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage37
Genome length (bp)59907
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionBenedictine University
Annotation StatusQC Complete
Plaque NotesThe plaque gave a bull's eye pattern (darker on the inside and lighter on the outside). After 24 hours of incubation the plaque diameter was 2 mm, and after both 48 and 72 hours of incubation the plaque diamater was 5 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Boiiii_1

Boiiii_2

Boiiii_3

Boiiii_4

Boiiii_6

Boiiii_7

Boiiii_8
(terminase)

Boiiii_9
(portal protein)

Boiiii_10
(capsid maturation protease)

Boiiii_11

Boiiii_12
(scaffolding protein)

Boiiii_13
(major capsid protein)

Boiiii_14
(head-to-tail adaptor)

Boiiii_15
(head-to-tail stopper)

Boiiii_16

Boiiii_17
(tail terminator)

Boiiii_18
(major tail protein)

Boiiii_19
(tail assembly chaperone)

Boiiii_20
(tail assembly chaperone)

Boiiii_21
(tape measure protein)

Boiiii_22
(minor tail protein)

Boiiii_23
(minor tail protein)

Boiiii_24
(minor tail protein)

Boiiii_25
(minor tail protein)

Boiiii_26
(minor tail protein)

Boiiii_27
(minor tail protein)

Boiiii_28
(minor tail protein)

Boiiii_29

Boiiii_30
(lysin A)

Boiiii_31
(lysin B)

Boiiii_32
(holin)

Boiiii_33

Boiiii_34

Boiiii_35
(MRE11 double strand break endo/exonuclease)

Boiiii_36
(helix-turn-helix DNA binding domain)

Boiiii_37

Boiiii_38

Boiiii_39

Boiiii_40

Boiiii_41
(tyrosine integrase)

Boiiii_42

Boiiii_43
(immunity repressor)

Boiiii_44
(Cro (control of repressor)

Boiiii_45
(excise)

Boiiii_46

Boiiii_47

Boiiii_48

Boiiii_49
(helix-turn-helix DNA binding protein, MerR-like)

Boiiii_50
(WhiB-family transcriptional factor)

Boiiii_51

Boiiii_52

Boiiii_53

Boiiii_54

Boiiii_55

Boiiii_56

Boiiii_57
(DnaE-like DNA Polymerse III (alpha))

Boiiii_58

Boiiii_59

Boiiii_60

Boiiii_61
(Cas4 family endonuclease)

Boiiii_62

Boiiii_63

Boiiii_64

Boiiii_65

Boiiii_66
(NrdH-like glutaredoxin)

Boiiii_67

Boiiii_68

Boiiii_69
(DNA primase/polymerase/helicase)

Boiiii_70

Boiiii_71
(RusA-like resolvase (endonuclease))

Boiiii_72

Boiiii_73

Boiiii_74

Boiiii_75

Boiiii_76
(SprT-like protease)

Boiiii_77

Boiiii_78

Boiiii_79

Boiiii_80

Boiiii_81

Boiiii_82

Boiiii_83

Boiiii_84

Boiiii_85
(rtcB-like RNA ligase)

Boiiii_86

Boiiii_87

Boiiii_88

Boiiii_89
(helix-turn-helix DNA binding domain)

Boiiii_90

Boiiii_91
(antitoxin in toxin/antitoxin system, HicB-like)

Boiiii_92
(toxin in toxin/antitoxin system, HicA-like)

Boiiii_93

Boiiii_94

Boiiii_95

Boiiii_96

Boiiii_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.10e10
Pitt Freezer Box# 7
Pitt Freezer Box Grid# E1
Available Files
GenBank File for PhameratorDownload