Mycobacterium phage Nibb
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Detailed Information for Phage Nibb
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlex Neitz and Vanessa Villela
Year Found2014
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates47.667233 N, 117.402333 W Map
Discovery NotesCollected after light rain, found 2 in deep in soil, date found 9/3/14 at 10:43 am, 11.11 degrees celius outside temperature
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1486
Genome length (bp)62293
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesSmall, circular, turbid on edges, and concentration dependent
MorphotypeSiphoviridae
Number of Genes102
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Nibb_1

Nibb_2

Nibb_3

Nibb_4 — tRNA

Nibb_5

Nibb_6

Nibb_7
(terminase)

Nibb_8
(portal protein)

Nibb_9
(MuF-like minor capsid protein)

Nibb_10

Nibb_11
(scaffolding protein)

Nibb_12
(major capsid protein)

Nibb_13
(head-to-tail adaptor)

Nibb_14
(head-to-tail stopper)

Nibb_15

Nibb_16
(tail terminator)

Nibb_17

Nibb_18

Nibb_19

Nibb_20
(tape measure protein)

Nibb_21
(minor tail protein)

Nibb_22
(minor tail protein)

Nibb_23
(minor tail protein)

Nibb_24
(minor tail protein)

Nibb_25
(minor tail protein)

Nibb_26
(minor tail protein)

Nibb_27
(minor tail protein)

Nibb_28

Nibb_29
(lysin A)

Nibb_30
(lysin B)

Nibb_31
(holin)

Nibb_32

Nibb_33

Nibb_34
(MRE11 double-strand break endo/exonuclease)

Nibb_35

Nibb_36

Nibb_37

Nibb_38

Nibb_39

Nibb_40

Nibb_41

Nibb_42
(queuine tRNA-ribosyltransferase)

Nibb_43
(tyrosine integrase)

Nibb_44

Nibb_45
(immunity repressor)

Nibb_46
(helix-turn-helix DNA binding protein)

Nibb_47
(excise)

Nibb_48

Nibb_49

Nibb_50

Nibb_51

Nibb_52

Nibb_53
(WhiB family transcription factor)

Nibb_54

Nibb_55

Nibb_56

Nibb_57

Nibb_58

Nibb_59
(DnaE-like DNA polymerase III)

Nibb_60

Nibb_61

Nibb_62
(Cas4 family endonuclease)

Nibb_63

Nibb_64

Nibb_65

Nibb_66

Nibb_67

Nibb_68

Nibb_69
(NrdH-like glutaredoxin)

Nibb_70
(HNH endonuclease)

Nibb_71
(DNA primase/helicase)

Nibb_72

Nibb_73
(RusA-like resolvase)

Nibb_74

Nibb_75

Nibb_76

Nibb_77

Nibb_78
(SprT-like protease)

Nibb_79

Nibb_80

Nibb_81

Nibb_82

Nibb_83

Nibb_84

Nibb_85

Nibb_86

Nibb_87
(RtcB-like RNA ligase)

Nibb_88

Nibb_89

Nibb_90

Nibb_91

Nibb_92

Nibb_93

Nibb_94

Nibb_95
(HicB-like antitoxin)

Nibb_96
(HicA-like toxin)

Nibb_97
(hydrolase)

Nibb_98

Nibb_99

Nibb_100

Nibb_101

Nibb_102

Nibb_103
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK460246
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.145e10
Date of SEA Lysate Titering Oct 16, 2014
Pitt Freezer Box# 16
Pitt Freezer Box Grid# H10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload