Mycobacterium phage Feyre
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Detailed Information for Phage Feyre
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTuesday Melton, Grace Renois
Year Found2021
Location FoundArkadelphia, AR United States
Finding InstitutionOuachita Baptist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.193387 N, 93.078221 W Map
Discovery NotesOur soil sample came from a compost pile in Arkadelphia, AR. After complementing 3 full plate titers, we concluded that we had a singular phage from our sample.
Naming NotesWe named our phage Feyre who is a very artistic and conflicted character. After exploring other phages, we found Rhysand who is very lytic. Rhysand and Feyre are married and it only made sense to name our beloved messy phage after her.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 12, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage628
Genome length (bp)63558
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content64.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesOur plaque showed a consistent size of about 0.4 cm through 3 full plate titers. For the most part, the plaques had a turbid look with blurred edges- only the occasional had a distinct outline. The centers were clear and became more turbid the farther from the center.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Feyre_1

Feyre_2

Feyre_3

Feyre_5

Feyre_6
(terminase)

Feyre_7
(portal protein)

Feyre_8
(capsid maturation protease)

Feyre_9

Feyre_10
(scaffolding protein)

Feyre_11
(major capsid protein)

Feyre_12
(head-to-tail adaptor)

Feyre_13
(head-to-tail stopper)

Feyre_14

Feyre_15
(tail terminator)

Feyre_16
(major tail protein)

Feyre_17
(tail assembly chaperone)

Feyre_18
(tail assembly chaperone)

Feyre_19
(tape measure protein)

Feyre_20
(minor tail protein)

Feyre_21
(minor tail protein)

Feyre_22
(minor tail protein)

Feyre_23
(minor tail protein)

Feyre_24
(minor tail protein)

Feyre_25
(minor tail protein)

Feyre_26

Feyre_27
(minor tail protein)

Feyre_28

Feyre_29
(lysin A)

Feyre_30
(lysin B)

Feyre_31
(holin)

Feyre_32

Feyre_33

Feyre_34
(phosphoesterase)

Feyre_35

Feyre_36

Feyre_37
(helix-turn-helix DNA binding protein)

Feyre_38
(serine integrase)

Feyre_39

Feyre_40

Feyre_41

Feyre_42
(transposase)

Feyre_43
(Cro protein)

Feyre_44

Feyre_45

Feyre_46

Feyre_47

Feyre_48
(DNA polymerase III sliding clamp beta)

Feyre_49

Feyre_50
(helix-turn-helix DNA binding domain protein)

Feyre_51

Feyre_52

Feyre_53

Feyre_54
(DnaQ-like DNA polymerase III subunit)

Feyre_55

Feyre_56

Feyre_57

Feyre_58
(Cas4 exonuclease)

Feyre_59

Feyre_60

Feyre_61

Feyre_62

Feyre_63
(NrdH-like glutaredoxin)

Feyre_64
(HNH endonuclease)

Feyre_65
(HNH endonuclease)

Feyre_66
(DNA primase/polymerase/helicase)

Feyre_67
(DNA helicase)

Feyre_68
(Holliday junction resolvase)

Feyre_69
(helix-turn-helix DNA binding domain protein)

Feyre_70

Feyre_71

Feyre_72
(helix-turn-helix DNA binding domain protein)

Feyre_73

Feyre_74
(helix-turn-helix DNA binding domain protein)

Feyre_75

Feyre_76

Feyre_77

Feyre_78

Feyre_79

Feyre_80
(ASCE ATPase)

Feyre_81

Feyre_82

Feyre_83

Feyre_84

Feyre_85

Feyre_86
(DpdA-like tRNA-guanine transglycosylase)

Feyre_87

Feyre_88

Feyre_89

Feyre_90
(BrnT-like toxin)

Feyre_91
(BrnA-like antitoxin)

Feyre_92

Feyre_93

Feyre_94

Feyre_95

Feyre_96
(helix-turn-helix DNA binding domain protein)

Feyre_97

Feyre_98
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP059644
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7 * 10^9 pfu/mL
Pitt Freezer Box# 135
Pitt Freezer Box Grid# F4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload