Mycobacterium phage Slimphazie
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Detailed Information for Phage Slimphazie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSlimphagie
Found ByAmara Ejikemeuwa and Bayla Bessemer
Year Found2015
Location FoundPensacola, FL USA
Finding InstitutionUniversity of West Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates30.551611 N, 87.218594 W Map
Discovery NotesThe soil was collected from the Camellia Garden at the University of West Florida.
Naming NotesThe phage was named after the rapper, Slim Shady (also known as Eminem).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 7, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2516
Genome length (bp)60143
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques were small, circular, and lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Slimphazie_1

Slimphazie_2

Slimphazie_3

Slimphazie_4

Slimphazie_6

Slimphazie_7

Slimphazie_8
(terminase)

Slimphazie_9
(portal protein)

Slimphazie_10
(MuF-like minor capsid protein)

Slimphazie_11

Slimphazie_12
(scaffolding protein)

Slimphazie_13
(major capsid protein)

Slimphazie_14
(head-to-tail adaptor)

Slimphazie_15
(head-to-tail stopper)

Slimphazie_16

Slimphazie_17
(tail terminator)

Slimphazie_18

Slimphazie_19

Slimphazie_20

Slimphazie_21
(tape measure protein)

Slimphazie_22
(minor tail protein)

Slimphazie_23
(minor tail protein)

Slimphazie_24
(minor tail protein)

Slimphazie_25
(minor tail protein)

Slimphazie_26
(minor tail protein)

Slimphazie_27
(minor tail protein)

Slimphazie_28
(minor tail protein)

Slimphazie_29

Slimphazie_30
(lysin A)

Slimphazie_31
(lysin B)

Slimphazie_32
(holin)

Slimphazie_33

Slimphazie_34

Slimphazie_35
(MRE11 double-strand break endo/exonuclease)

Slimphazie_36

Slimphazie_37

Slimphazie_38

Slimphazie_39

Slimphazie_40

Slimphazie_41
(tyrosine integrase)

Slimphazie_42

Slimphazie_43
(immunity repressor)

Slimphazie_44
(helix-turn-helix DNA binding protein)

Slimphazie_45
(excise)

Slimphazie_46

Slimphazie_47

Slimphazie_48

Slimphazie_49
(MerR-like helix-turn-helix DNA binding domain protein)

Slimphazie_50
(WhiB family transcription factor)

Slimphazie_51

Slimphazie_52

Slimphazie_53

Slimphazie_54

Slimphazie_55

Slimphazie_56

Slimphazie_57
(DnaE-like DNA polymerase III)

Slimphazie_58

Slimphazie_59

Slimphazie_60

Slimphazie_61
(Cas4 family endonuclease)

Slimphazie_62

Slimphazie_63

Slimphazie_64

Slimphazie_65

Slimphazie_66

Slimphazie_67
(NrdH-like glutaredoxin)

Slimphazie_68

Slimphazie_69

Slimphazie_70
(DNA primase/helicase)

Slimphazie_71

Slimphazie_72
(RusA-like resolvase)

Slimphazie_73

Slimphazie_74

Slimphazie_75

Slimphazie_76

Slimphazie_77

Slimphazie_78
(SprT-like protease)

Slimphazie_79

Slimphazie_80

Slimphazie_81

Slimphazie_82

Slimphazie_83

Slimphazie_84

Slimphazie_85

Slimphazie_86

Slimphazie_87
(RtcB-like RNA ligase)

Slimphazie_88

Slimphazie_89

Slimphazie_90

Slimphazie_91

Slimphazie_92

Slimphazie_93
(HicB-like antitoxin)

Slimphazie_94

Slimphazie_95

Slimphazie_96

Slimphazie_97

Slimphazie_98

Slimphazie_99
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140428
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Archived
Pitt Freezer Box# 18
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload