Mycobacterium phage Wintermute
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Detailed Information for Phage Wintermute
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBryan Pasqualucci
Year Found2015
Location FoundAnsonia, CT USA
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature42°C
GPS Coordinates41.346817 N, 73.077578 W Map
Discovery NotesThe sample was collected from underneath a cucumber plant during heavy rain. The air temperature was 23.3 degrees Celsius. The garden is regularly planted in and fruit was growing from the plant.
Naming NotesWintermute is a character from William S. Gibson's novel Neuromancer. Wintermute is one of a pair of AI's who is trying to merge with the other to become a super-intelligence. It is one of my favorite books and I liked that the character is trying to integrate itself into something just like my temperate phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage42
Genome length (bp)58046
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content68.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesGenerally plaques were 3mm in diameter. Appearing with a 2mm clear center and 1mm fuzzy ring. As plaque density increased as with web plates, they plaques were generally closer to 2mm.
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Wintermute_1

Wintermute_2

Wintermute_3

Wintermute_4

Wintermute_5

Wintermute_6

Wintermute_7

Wintermute_8

Wintermute_9
(terminase)

Wintermute_10
(portal protein)

Wintermute_11
(MuF-like minor capsid protein)

Wintermute_12

Wintermute_13
(scaffolding protein)

Wintermute_14
(major capsid protein)

Wintermute_15
(head-to-tail adaptor)

Wintermute_16
(head-to-tail stopper)

Wintermute_17

Wintermute_18
(tail terminator)

Wintermute_19
(major tail protein)

Wintermute_20
(tail assembly chaperone)

Wintermute_21
(tail assembly chaperone)

Wintermute_22
(tape measure protein)

Wintermute_23
(minor tail protein)

Wintermute_24
(minor tail protein)

Wintermute_25
(minor tail protein)

Wintermute_26
(minor tail protein)

Wintermute_27
(minor tail protein)

Wintermute_28
(minor tail protein)

Wintermute_29
(minor tail protein)

Wintermute_30

Wintermute_31
(lysin A)

Wintermute_32
(lysin B)

Wintermute_33
(holin)

Wintermute_34

Wintermute_35

Wintermute_36
(MRE11 double-strand break endo/exonuclease)

Wintermute_37

Wintermute_38 — tRNA

Wintermute_39
(LamD-like)

Wintermute_40

Wintermute_41

Wintermute_42

Wintermute_43

Wintermute_44

Wintermute_45
(tyrosine integrase)

Wintermute_46

Wintermute_47
(immunity repressor)

Wintermute_48
(Cro protein)

Wintermute_49
(excise)

Wintermute_50

Wintermute_51

Wintermute_52

Wintermute_53

Wintermute_54

Wintermute_55

Wintermute_56

Wintermute_57

Wintermute_58

Wintermute_59

Wintermute_60

Wintermute_61

Wintermute_62
(DnaQ-like DNA polymerase III subunit)

Wintermute_63

Wintermute_64
(Cas4 family exonuclease)

Wintermute_65

Wintermute_66

Wintermute_67

Wintermute_68

Wintermute_69

Wintermute_70
(NrdH-like glutaredoxin)

Wintermute_71
(methyltransferase)

Wintermute_72

Wintermute_73
(HNH endonuclease)

Wintermute_74
(DNA primase)

Wintermute_75

Wintermute_76
(HNH endonuclease)

Wintermute_77

Wintermute_78

Wintermute_79

Wintermute_80

Wintermute_81
(SprT-like protease)

Wintermute_82

Wintermute_83

Wintermute_84

Wintermute_85

Wintermute_86

Wintermute_87

Wintermute_88

Wintermute_89

Wintermute_90

Wintermute_91

Wintermute_92

Wintermute_93

Wintermute_94
(HNH endonuclease)

Wintermute_95
(methyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140435
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.2x10^10
Date of SEA Lysate Titering Oct 28, 2015
Pitt Freezer Box# 23
Pitt Freezer Box Grid# H10
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload