Mycobacterium phage BaghaKamala
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Detailed Information for Phage BaghaKamala
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJulie Isola
Year Found2015
Location FoundAllegan, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature22°C
GPS Coordinates42.500833 N, 85.810167 W Map
Discovery NotesThe dirt was taken from next to a rotting tree stump. The soil was very moist and it was taken at a depth of 2.5 inches.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 1, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage235
Genome length (bp)59132
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUniversity of Massachusetts Amherst
Annotation StatusIn GenBank
Plaque NotesThe plaques appeared at 48hrs. They are about 3mm in diameter and they are round with fuzzy edges and clear centers. The morphology did have a bullseye to it but it faded away while it was being purified.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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BaghaKamala_1

BaghaKamala_2

BaghaKamala_3
(ATPase domain)

BaghaKamala_4

BaghaKamala_6

BaghaKamala_7

BaghaKamala_8
(terminase)

BaghaKamala_9
(portal protein)

BaghaKamala_10
(MuF-like minor capsid protein)

BaghaKamala_11

BaghaKamala_12
(scaffolding protein)

BaghaKamala_13
(major capsid protein)

BaghaKamala_14
(head-to-tail adaptor)

BaghaKamala_15
(head-to-tail stopper)

BaghaKamala_16

BaghaKamala_17
(tail terminator)

BaghaKamala_18
(major tail protein)

BaghaKamala_19
(tail assembly chaperone)

BaghaKamala_20
(tail assembly chaperone)

BaghaKamala_21
(tape measure protein)

BaghaKamala_22
(minor tail protein)

BaghaKamala_23
(minor tail protein)

BaghaKamala_24

BaghaKamala_25
(minor tail protein)

BaghaKamala_26

BaghaKamala_27
(minor tail protein)

BaghaKamala_28
(minor tail protein)

BaghaKamala_29

BaghaKamala_30
(lysin A)

BaghaKamala_31
(lysin B)

BaghaKamala_32
(holin)

BaghaKamala_33

BaghaKamala_34

BaghaKamala_35
(exonuclease)

BaghaKamala_36
(helix-turn-helix DNA binding domain protein)

BaghaKamala_37

BaghaKamala_38

BaghaKamala_39

BaghaKamala_40
(tyrosine integrase)

BaghaKamala_41

BaghaKamala_42
(immunity repressor)

BaghaKamala_43
(helix-turn-helix DNA binding protein)

BaghaKamala_44
(excise)

BaghaKamala_45

BaghaKamala_46

BaghaKamala_47

BaghaKamala_48
(helix-turn-helix DNA binding domain)

BaghaKamala_49
(WhiB family transcription factor)

BaghaKamala_50

BaghaKamala_51

BaghaKamala_52

BaghaKamala_53

BaghaKamala_54

BaghaKamala_55

BaghaKamala_56
(DnaQ-like (DNA polymerase III subunit))

BaghaKamala_57

BaghaKamala_58

BaghaKamala_59

BaghaKamala_60
(exonuclease)

BaghaKamala_61

BaghaKamala_62

BaghaKamala_63

BaghaKamala_64

BaghaKamala_65

BaghaKamala_66
(NrdH-like glutaredoxin)

BaghaKamala_67
(HNH endonuclease)

BaghaKamala_68
(DNA primase)

BaghaKamala_69

BaghaKamala_70
(RusA-like resolvase (endonuclease))

BaghaKamala_71

BaghaKamala_72
(helix-turn-helix DNA binding domain)

BaghaKamala_73

BaghaKamala_74

BaghaKamala_75
(SprT-like protease)

BaghaKamala_76

BaghaKamala_77

BaghaKamala_78
(helix-turn-helix DNA binding domain)

BaghaKamala_79

BaghaKamala_80

BaghaKamala_81

BaghaKamala_82

BaghaKamala_83

BaghaKamala_84
(RtcB-like RNA ligase)

BaghaKamala_85

BaghaKamala_86

BaghaKamala_87

BaghaKamala_88

BaghaKamala_89

BaghaKamala_90
(antitoxin in toxin/antitoxin system, HicB-like)

BaghaKamala_91
(toxin in toxin/antitoxin system, HicA-like)

BaghaKamala_92

BaghaKamala_93

BaghaKamala_94

BaghaKamala_95

BaghaKamala_96
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW712730
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.65x10^9
Pitt Freezer Box# 19
Pitt Freezer Box Grid# D4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload