Mycobacterium phage Rosmarinus
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Detailed Information for Phage Rosmarinus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCoral Ghrist
Year Found2021
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.984722 N, 86.455278 W Map
Discovery NotesThe soil was dark brown and damp. The soil was surrounded by organic matter of moss and leaves. The area was shaded and sheltered by a bridge. The depth collected at was two centimeters below the surface. The ambient temperature was 25 degrees celsius.
Naming NotesThe name originated from the spice rosemary. Rosemary looks similar to the bacteriophage because like the bacteriophage it is composed of several small pieces.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1225
Genome length (bp)59696
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesThe plaques are turbid which implies that the phage is temperate. The plaques are 2mm. The plaques have a halo effect on the outside rim.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rosmarinus_1

Rosmarinus_2

Rosmarinus_3

Rosmarinus_4

Rosmarinus_6

Rosmarinus_7

Rosmarinus_8
(terminase)

Rosmarinus_9
(portal protein)

Rosmarinus_10
(capsid accessory protein)

Rosmarinus_11

Rosmarinus_12
(scaffolding protein)

Rosmarinus_13
(major capsid protein)

Rosmarinus_14
(head-to-tail adaptor)

Rosmarinus_15
(head-to-tail stopper)

Rosmarinus_16

Rosmarinus_17
(tail terminator)

Rosmarinus_18
(major tail protein)

Rosmarinus_19
(tail assembly chaperone)

Rosmarinus_20
(tail assembly chaperone)

Rosmarinus_21
(tape measure protein)

Rosmarinus_22
(minor tail protein)

Rosmarinus_23
(minor tail protein)

Rosmarinus_24

Rosmarinus_25

Rosmarinus_26

Rosmarinus_27
(minor tail protein)

Rosmarinus_28

Rosmarinus_29

Rosmarinus_30
(lysin A)

Rosmarinus_31
(lysin B)

Rosmarinus_32
(holin)

Rosmarinus_33

Rosmarinus_34

Rosmarinus_35
(MRE11 double-strand break endo/exonuclease)

Rosmarinus_36
(helix-turn-helix DNA binding domain)

Rosmarinus_37

Rosmarinus_38

Rosmarinus_39

Rosmarinus_40

Rosmarinus_41
(tyrosine integrase)

Rosmarinus_42

Rosmarinus_43
(immunity repressor)

Rosmarinus_44
(Cro (control of repressor)

Rosmarinus_45
(excise)

Rosmarinus_46

Rosmarinus_47

Rosmarinus_48

Rosmarinus_49

Rosmarinus_50
(WhiB family transcription factor)

Rosmarinus_51

Rosmarinus_52

Rosmarinus_53

Rosmarinus_54

Rosmarinus_55

Rosmarinus_56

Rosmarinus_57
(exonuclease)

Rosmarinus_58

Rosmarinus_59

Rosmarinus_60

Rosmarinus_61
(Cas4 family exonuclease)

Rosmarinus_62

Rosmarinus_63

Rosmarinus_64

Rosmarinus_65

Rosmarinus_66
(NrdH-like glutaredoxin)

Rosmarinus_67

Rosmarinus_68
(HNH endonuclease)

Rosmarinus_69
(DNA primase/helicase)

Rosmarinus_70

Rosmarinus_71
(RusA-like resolvase (endonuclease))

Rosmarinus_72

Rosmarinus_73

Rosmarinus_74

Rosmarinus_75

Rosmarinus_76
(SprT-like protease)

Rosmarinus_77

Rosmarinus_78

Rosmarinus_79

Rosmarinus_80

Rosmarinus_81
(helix-turn-helix DNA binding domain)

Rosmarinus_82

Rosmarinus_83

Rosmarinus_84

Rosmarinus_85
(rtcB-like RNA ligase)

Rosmarinus_86

Rosmarinus_87

Rosmarinus_88

Rosmarinus_89

Rosmarinus_90

Rosmarinus_91
(antitoxin in toxin/antitoxin system, HicB-like)

Rosmarinus_92
(toxin in toxin/antitoxin system, HicA-like)

Rosmarinus_93

Rosmarinus_94

Rosmarinus_95

Rosmarinus_96

Rosmarinus_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456353
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2 x 10^9
Pitt Freezer Box# 137
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload