Detailed Information for Phage Atiba |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Shelby Hartley |
Year Found | 2016 |
Location Found | Hattiesburg, MS USA |
Finding Institution | University of Southern Mississippi |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Unknown |
Isolation Temperature | Not entered |
GPS Coordinates | 31.32903 N, 89.333516 W Map |
Discovery Notes | I found my phage underneath a patch of leaves which was shaded by trees. The soil was damp with lots of debris in it such as twigs and leaves. To collect my soil sample, I moved the leaves and collected the soil directly under the leaves. |
Naming Notes | I named my phage Atiba after a horse that I used to ride at a summer camp I attended as a child. This horse was there every year and he was my favorite horse to ride. I like his color which reminds of cookies and cream. Also, I just like his name and think it is unique. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 10, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 599 |
Genome length (bp) | 59556 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CTCGTAGGCAT |
GC Content | 66.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | My plaques were around 6mm in diameter, turbid and circular. Some had bullseyes in the center. All of my plaques were around the same size. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN234230 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
33 |
Pitt Freezer Box Grid# |
H1 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |