Mycobacterium phage Heftyboy
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Detailed Information for Phage Heftyboy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTaylor Hefty and Alexa Salvador
Year Found2014
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.666811 N, 117.404644 W Map
Discovery NotesSoil came from around a tree on the Gonzaga University Campus, the dirt had pine needles and little rocks around the given soil. The weather on the day we collected our sample was cold and damp, approximately what 45 degrees fahrenheit.
Naming NotesThe name came from my last name and the phage was definitely a little hefty.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 7, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1013
Genome length (bp)61968
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesThe plaques were very clear. The plaques appear very round and the diameter of each plaque is about 3.9 mm.
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Heftyboy_1

Heftyboy_2

Heftyboy_3

Heftyboy_4 — tRNA
(tRNA-Trp(cca))

Heftyboy_5

Heftyboy_6
(terminase)

Heftyboy_7
(portal protein)

Heftyboy_8
(capsid maturation protease)

Heftyboy_9

Heftyboy_10
(scaffolding protein)

Heftyboy_11
(major capsid protein)

Heftyboy_12
(head-to-tail adaptor)

Heftyboy_13
(head-to-tail stopper)

Heftyboy_14

Heftyboy_15
(tail terminator)

Heftyboy_16
(major tail protein)

Heftyboy_17
(tail assembly chaperone)

Heftyboy_18
(tail assembly chaperone)

Heftyboy_19
(tape measure protein)

Heftyboy_20
(minor tail protein)

Heftyboy_21
(minor tail protein)

Heftyboy_22

Heftyboy_23
(minor tail protein)

Heftyboy_24

Heftyboy_25
(minor tail protein)

Heftyboy_26

Heftyboy_27
(minor tail protein)

Heftyboy_28

Heftyboy_29
(lysin A)

Heftyboy_30
(lysin B)

Heftyboy_31
(holin)

Heftyboy_32

Heftyboy_33

Heftyboy_34
(metallophosphatase)

Heftyboy_35

Heftyboy_36

Heftyboy_37
(helix-turn-helix DNA binding domain protein)

Heftyboy_38
(serine integrase)

Heftyboy_39

Heftyboy_40

Heftyboy_41
(immunity repressor)

Heftyboy_42
(helix-turn-helix DNA binding domain protein)

Heftyboy_43

Heftyboy_44

Heftyboy_45

Heftyboy_46

Heftyboy_47
(DNA polymerase III sliding clamp beta)

Heftyboy_48

Heftyboy_49

Heftyboy_50

Heftyboy_51

Heftyboy_52

Heftyboy_53
(DnaQ-like DNA polymerase III subunit)

Heftyboy_54

Heftyboy_55

Heftyboy_56
(exonuclease)

Heftyboy_57

Heftyboy_58

Heftyboy_59

Heftyboy_60

Heftyboy_61
(NrdH-like glutaredoxin)

Heftyboy_62
(HNH endonuclease)

Heftyboy_63
(HNH endonuclease)

Heftyboy_64
(DNA primase/polymerase)

Heftyboy_65
(DNA helicase)

Heftyboy_66
(Holliday junction resolvase)

Heftyboy_67

Heftyboy_68

Heftyboy_69

Heftyboy_70

Heftyboy_71

Heftyboy_72

Heftyboy_73

Heftyboy_74

Heftyboy_75

Heftyboy_76

Heftyboy_77

Heftyboy_78

Heftyboy_79

Heftyboy_80

Heftyboy_81

Heftyboy_82

Heftyboy_83

Heftyboy_84

Heftyboy_85

Heftyboy_86
(DpdA-like tRNA-guanine transglycosylase)

Heftyboy_87

Heftyboy_88
(BrnT-like toxin)

Heftyboy_89
(BrnA-like antitoxin)

Heftyboy_90

Heftyboy_91

Heftyboy_92

Heftyboy_93

Heftyboy_94

Heftyboy_95

Heftyboy_96
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR475256
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload