| Detailed Information for Phage Ximenita |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Monica Elliott |
| Year Found | 2014 |
| Location Found | Fort Collins, CO USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 40.511944 N, 105.065 W Map |
| Discovery Notes | The soil was collected on August 20, 2014 at 9:08 pm. The air temperature at collection was 22.2˚C. The soil was fairly dry, and it was extracted from a 5cm deep hole near the roots of a large pine tree. The surrounding area was well-shaded, and dead leaves covered the ground |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 30, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2662 |
| Genome length (bp) | 61027 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCGGGGCAT |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | K |
| Subcluster | K6 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | N/A |
| Other Cluster Members |
|
| Annotating Institution | Rockland Community College |
| Annotation Status | Being Annotated (Expected completion by 6/30/2019) |
| Plaque Notes | Plaques were circular and turbid when grown at 32ºC. Over time, they grew larger in diameter, and as the plaques grew, the edges became more defined, and a more turbid center developed. Plaque size growth over time was observed consistently, and new plaques would also continue to form and develop as time passed.
When incubated at 42℃, no plaques grew. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.6 x 10^9 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
