The Actinobacteriophage Database

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Detailed Information for Phage Ximenita
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Monica Elliott
Year Found	2014
Location Found	Fort Collins, CO USA
Finding Institution	Hope College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	32°C
GPS Coordinates	40.511944 N, 105.065 W Map
Discovery Notes	The soil was collected on August 20, 2014 at 9:08 pm. The air temperature at collection was 22.2˚C. The soil was fairly dry, and it was extracted from a 5cm deep hole near the roots of a large pine tree. The surrounding area was well-shaded, and dead leaves covered the ground
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Sep 30, 2015
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	2662
Genome length (bp)	61027
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CTCGGGGCAT
GC Content	66.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K6
Cluster Life Cycle	Temperate
Lysogeny Notes	N/A
Other Cluster Members	Click to View Nergal (None) ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) Capricorn (K1) CaseJules (K1) CheetoDust (K1) Chris (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Lunahalos (K1) MarkPhew (K1) Mdavu (K1) MeaningOfLife (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) TiniBug (K1) Tiri (K1) Topper (K1) TreyKay (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) MacnCheese (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Boilgate (K4) Chancellor (K4) Cheetobro (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7)
Annotating Institution	Rockland Community College
Annotation Status	In GenBank
Plaque Notes	Plaques were circular and turbid when grown at 32ºC. Over time, they grew larger in diameter, and as the plaques grew, the edges became more defined, and a more turbid center developed. Plaque size growth over time was observed consistently, and new plaques would also continue to form and develop as time passed. When incubated at 42℃, no plaques grew.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Ximenita_1 Ximenita_2 Ximenita_3 Ximenita_5 Ximenita_6 Ximenita_7 Ximenita_8 (terminase) Ximenita_9 (portal protein) Ximenita_10 (MuF-like minor capsid protein) Ximenita_11 Ximenita_12 (scaffolding protein) Ximenita_13 (major capsid protein) Ximenita_14 (head-to-tail adaptor) Ximenita_15 (head-to-tail stopper) Ximenita_16 Ximenita_17 (tail terminator) Ximenita_18 (major tail protein) Ximenita_19 (tail assembly chaperone) Ximenita_20 (tail assembly chaperone) Ximenita_21 (tape measure protein) Ximenita_22 (minor tail protein) Ximenita_23 (minor tail protein) Ximenita_24 Ximenita_25 (minor tail protein) Ximenita_26 Ximenita_27 (minor tail protein) Ximenita_28 Ximenita_29 (minor tail protein) Ximenita_30 Ximenita_31 (lysin A) Ximenita_32 (lysin B) Ximenita_33 (holin) Ximenita_34 Ximenita_35 Ximenita_36 (MRE11 double-strand break endo/exonuclease) Ximenita_37 Ximenita_38 Ximenita_39 Ximenita_40 Ximenita_41 Ximenita_42 Ximenita_43 Ximenita_44 Ximenita_45 Ximenita_46 (DNA binding protein) Ximenita_47 Ximenita_48 Ximenita_49 (immunity repressor) Ximenita_50 (Cro protein) Ximenita_51 (excise) Ximenita_52 Ximenita_53 Ximenita_54 Ximenita_55 Ximenita_56 Ximenita_57 (WhiB family transcription factor) Ximenita_58 Ximenita_59 Ximenita_60 Ximenita_61 Ximenita_62 Ximenita_63 (DNA polymerase II exonuclease) Ximenita_64 Ximenita_65 (Cas4 family exonuclease) Ximenita_66 Ximenita_67 Ximenita_68 Ximenita_69 Ximenita_70 Ximenita_71 Ximenita_72 (NrdH-like glutaredoxin) Ximenita_73 (HNH endonuclease) Ximenita_74 (DNA primase/helicase) Ximenita_75 Ximenita_76 (RusA-like resolvase) Ximenita_77 Ximenita_78 Ximenita_79 Ximenita_80 Ximenita_81 Ximenita_82 Ximenita_83 Ximenita_84 (SprT-like protease) Ximenita_85 Ximenita_86 Ximenita_87 Ximenita_88 Ximenita_89 Ximenita_90 Ximenita_91 (RtcB-like RNA ligase) Ximenita_92 Ximenita_93 Ximenita_94 Ximenita_95 Ximenita_96 Ximenita_97 Ximenita_98 Ximenita_99 Ximenita_100 Ximenita_101 Ximenita_102 Ximenita_103 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN945901
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	9.6 x 10^9
Pitt Freezer Box#	7
Pitt Freezer Box Grid#	G10
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
GenBank File for Phamerator	Download

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