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Mycobacterium phage Sulley
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Detailed Information for Phage Sulley
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam FAV
Year Found2013
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.07359 N, 118.453065 W Map
Discovery NotesPhage Sulley was isolated from a sample taken on April 2nd, 2013 a grassy field in Sycamore Park at UCLA in Los Angeles, CA. Isolation, characterization and annotation of Phage Sulley was done by Team Florence and the Vaccine (Katelynn Kazane, Annie Chen, Yousif Ketoola, Emily Lundberg). Annotation quality checked by Yousif Ketoola. TA: Drake Williams. Instructor: Jordan Moberg Parker. Laboratory Support: Krisanavane Reddi.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 8, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage80
Genome length (bp)59873
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesClear plaques, genome contains integrase.
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are clear with a hazy border. Average 3mm, but range from 2.5-5 mm in diameter.
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs1
Has been Phamerated?Yes
Gene List

Click to View

Sulley_1

Sulley_2

Sulley_3

Sulley_4

Sulley_6

Sulley_7

Sulley_8
(terminase)

Sulley_9
(portal protein)

Sulley_10
(MuF-like minor capsid protein)

Sulley_11

Sulley_12
(scaffolding protein)

Sulley_13
(major capsid protein)

Sulley_14
(head-to-tail adaptor)

Sulley_15
(head-to-tail stopper)

Sulley_16

Sulley_17
(tail terminator)

Sulley_18

Sulley_19

Sulley_20

Sulley_21
(tape measure protein)

Sulley_22
(minor tail protein)

Sulley_23
(minor tail protein)

Sulley_24
(minor tail protein)

Sulley_25
(minor tail protein)

Sulley_26
(minor tail protein)

Sulley_27
(minor tail protein)

Sulley_28
(minor tail protein)

Sulley_29

Sulley_30
(lysin A)

Sulley_31
(lysin B)

Sulley_32
(holin)

Sulley_33

Sulley_34

Sulley_35
(MRE11 double-strand break endo/exonuclease)

Sulley_36

Sulley_37

Sulley_38

Sulley_39

Sulley_40

Sulley_41
(tyrosine integrase)

Sulley_42

Sulley_43
(immunity repressor)

Sulley_44
(helix-turn-helix DNA binding protein)

Sulley_45
(excise)

Sulley_46

Sulley_47

Sulley_48

Sulley_49
(MerR-like helix-turn-helix DNA binding domain protein)

Sulley_50
(WhiB family transcription factor)

Sulley_51

Sulley_52

Sulley_53

Sulley_54

Sulley_55

Sulley_56

Sulley_57
(DnaE-like DNA polymerase III)

Sulley_58

Sulley_59

Sulley_60

Sulley_61
(Cas4 family endonuclease)

Sulley_62

Sulley_63

Sulley_64

Sulley_65

Sulley_66
(NrdH-like glutaredoxin)

Sulley_67
(HNH endonuclease)

Sulley_68
(DNA primase/helicase)

Sulley_69
(RusA-like resolvase)

Sulley_70

Sulley_71

Sulley_72

Sulley_73

Sulley_74

Sulley_75

Sulley_76

Sulley_77

Sulley_78

Sulley_79

Sulley_80

Sulley_81

Sulley_82
(RtcB-like RNA ligase)

Sulley_83

Sulley_84

Sulley_85

Sulley_86

Sulley_87

Sulley_88
(HicB-like antitoxin)

Sulley_89
(HicA-like toxin)

Sulley_90

Sulley_91

Sulley_92

Sulley_93

Sulley_94

Sulley_95
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919532
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload