Mycobacterium phage Mynx
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Detailed Information for Phage Mynx
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPaige Stone, Lori Callnan
Year Found2015
Location FoundBucksport, ME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.644444 N, 68.800833 W Map
Discovery NotesSoil came from a garden of potatoes with bacterial infection. Garden was next to an old turkey pen.
Naming NotesThe name of this phage came from the characteristics of the phage. The phage is unpredictable and can be very frustrating at times.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 17, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2423
Genome length (bp)60055
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesPlaques are very small and clear at high concentrations. As the concentration lowers, the plaques get continuously more turbid and larger. At lower concentrations the plaques usually have a clear dot in the middle with a turbid ring around it.
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Mynx_1

Mynx_2

Mynx_3

Mynx_4

Mynx_5 — tRNA
(tRNA-Trp(cca))

Mynx_6

Mynx_7

Mynx_8
(terminase)

Mynx_9
(portal protein)

Mynx_10
(MuF-like minor capsid protein)

Mynx_11

Mynx_12
(scaffolding protein)

Mynx_13
(major capsid protein)

Mynx_14
(head-to-tail adaptor)

Mynx_15
(head-to-tail stopper)

Mynx_16

Mynx_17
(tail terminator)

Mynx_18

Mynx_19

Mynx_20

Mynx_21
(tape measure protein)

Mynx_22
(minor tail protein)

Mynx_23
(minor tail protein)

Mynx_24
(minor tail protein)

Mynx_25
(minor tail protein)

Mynx_26

Mynx_27
(minor tail protein)

Mynx_28
(minor tail protein)

Mynx_29

Mynx_30
(lysin A)

Mynx_31
(lysin B)

Mynx_32
(holin)

Mynx_33

Mynx_34

Mynx_35
(MRE11 double-strand break endo/exonuclease)

Mynx_36

Mynx_37

Mynx_38

Mynx_39

Mynx_40
(queuine tRNA-ribosyltransferase)

Mynx_41

Mynx_42
(tyrosine integrase)

Mynx_43

Mynx_44
(immunity repressor)

Mynx_45
(helix-turn-helix DNA binding protein)

Mynx_46
(excise)

Mynx_47

Mynx_48

Mynx_49

Mynx_50
(MerR-like helix-turn-helix DNA binding domain protein)

Mynx_51
(WhiB family transcription factor)

Mynx_52

Mynx_53

Mynx_54

Mynx_55

Mynx_56

Mynx_57

Mynx_58
(DnaE-like DNA polymerase III)

Mynx_59

Mynx_60

Mynx_61

Mynx_62
(Cas4 family endonuclease)

Mynx_63

Mynx_64

Mynx_65

Mynx_66

Mynx_67

Mynx_68
(NrdH-like glutaredoxin)

Mynx_69

Mynx_70
(HNH endonuclease)

Mynx_71
(DNA primase/helicase)

Mynx_72
(RusA-like resolvase)

Mynx_73

Mynx_74

Mynx_75

Mynx_76

Mynx_77

Mynx_78
(SprT-like protease)

Mynx_79

Mynx_80

Mynx_81

Mynx_82

Mynx_83

Mynx_84

Mynx_85

Mynx_86
(RtcB-like RNA ligase)

Mynx_87
(DNA binding protein)

Mynx_88

Mynx_89

Mynx_90

Mynx_91

Mynx_92
(HicB-like antitoxin)

Mynx_93
(HicA-like toxin)

Mynx_94

Mynx_95

Mynx_96

Mynx_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH513977
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.16*10^9
Date of SEA Lysate Titering Nov 10, 2015
Pitt Freezer Box# 23
Pitt Freezer Box Grid# B1
Available Files
EM PictureDownload
GenBank File for PhameratorDownload