Mycobacterium phage Jeckyll
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Detailed Information for Phage Jeckyll
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTaylor Senay
Year Found2015
Location FoundElizabethtown, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates37.725833 N, 85.862778 W Map
Discovery NotesSoil sample was obtained from an area about a meter from the walking/biking trail with light foot traffic. The soil itself was dry, taken from about 5cm under a fallen tree limb. The surrounding area was wooded, with no buildings or concrete in sight. Sample was taken from an area approximately 30m from a small standing creek. Air temperature was approximately 30 degrees Celsius.
Naming NotesThis phage exhibited particularly aggressive clear plaque mutants. This brought to mind the story of Dr. Jekyll and Mr. Hyde. Since the name Jekyll was already taken, I opted for a slight alteration in spelling.

See Sequencing Notes below for information about the sequencing of one of these clear plaque mutants.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 5, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage350
Genome length (bp)59708
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Sequencing NotesA clear plaque mutant of Jeckyll was isolated and sequenced. It had two differences from the wild type Jeckyll.

G instead of A at position 34912.

G instead of A at position 41250.
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesTemperate phage
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesTurbid plaques, typically 3-4mm in diameter, with small clear centers. Incubated at 37˚C for 48 hours to produce plaques this size
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Jeckyll_1

Jeckyll_2

Jeckyll_3

Jeckyll_4

Jeckyll_6

Jeckyll_7

Jeckyll_8
(terminase)

Jeckyll_9
(portal protein)

Jeckyll_10
(MuF-like minor capsid protein)

Jeckyll_11

Jeckyll_12
(scaffolding protein)

Jeckyll_13
(major capsid protein)

Jeckyll_14
(head-to-tail adaptor)

Jeckyll_15
(head-to-tail stopper)

Jeckyll_16

Jeckyll_17
(tail terminator)

Jeckyll_18

Jeckyll_19

Jeckyll_20

Jeckyll_21
(tape measure protein)

Jeckyll_22
(minor tail protein)

Jeckyll_23
(minor tail protein)

Jeckyll_24
(minor tail protein)

Jeckyll_25
(minor tail protein)

Jeckyll_26
(minor tail protein)

Jeckyll_27
(minor tail protein)

Jeckyll_28
(minor tail protein)

Jeckyll_29

Jeckyll_30
(lysin A)

Jeckyll_31
(lysin B)

Jeckyll_32
(holin)

Jeckyll_33

Jeckyll_34

Jeckyll_35
(MRE11 double-strand break endo/exonuclease)

Jeckyll_36

Jeckyll_37

Jeckyll_38

Jeckyll_39

Jeckyll_40

Jeckyll_41
(tyrosine integrase)

Jeckyll_42

Jeckyll_43
(immunity repressor)

Jeckyll_44
(helix-turn-helix DNA binding protein)

Jeckyll_45
(excise)

Jeckyll_46

Jeckyll_47

Jeckyll_48

Jeckyll_49
(MerR-like helix-turn-helix DNA binding domain protein)

Jeckyll_50
(WhiB family transcription factor)

Jeckyll_51

Jeckyll_52

Jeckyll_53

Jeckyll_54

Jeckyll_55

Jeckyll_56

Jeckyll_57
(DnaE-like DNA polymerase III)

Jeckyll_58

Jeckyll_59

Jeckyll_60

Jeckyll_61
(Cas4 family endonuclease)

Jeckyll_62

Jeckyll_63

Jeckyll_64

Jeckyll_65

Jeckyll_66
(NrdH-like glutaredoxin)

Jeckyll_67

Jeckyll_68
(HNH endonuclease)

Jeckyll_69
(DNA primase/helicase)

Jeckyll_70

Jeckyll_71
(RusA-like resolvase)

Jeckyll_72

Jeckyll_73

Jeckyll_74

Jeckyll_75

Jeckyll_76
(SprT-like protease)

Jeckyll_77

Jeckyll_78

Jeckyll_79

Jeckyll_80

Jeckyll_81

Jeckyll_82

Jeckyll_83

Jeckyll_84

Jeckyll_85
(RtcB-like RNA ligase)

Jeckyll_86

Jeckyll_87

Jeckyll_88

Jeckyll_89

Jeckyll_90

Jeckyll_91
(HicB-like antitoxin)

Jeckyll_92

Jeckyll_93

Jeckyll_94

Jeckyll_95

Jeckyll_96

Jeckyll_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140412
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.4x10^8
Pitt Freezer Box# 27
Pitt Freezer Box Grid# E11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload