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Mycobacterium phage Beezoo
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Detailed Information for Phage Beezoo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBrian Zhu
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.93262 N, 86.40674 W Map
Discovery Notes-From a compost pile
-24 degrees Celsius
-8 cm digging depth
-Sample collected on 09/20/2016
Naming NotesThe phage name is the phonetics of the phage finder's first initial and last name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 27, 2017
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage320
Genome length (bp)60494
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

Tiri (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque Notes-Turbid plaques.
-3.2 mm
-Incubated for 48 hours at 30 degrees Celsius
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Beezoo_1

Beezoo_2

Beezoo_3

Beezoo_4

Beezoo_6

Beezoo_7

Beezoo_8
(terminase)

Beezoo_9
(portal protein)

Beezoo_10
(MuF-like minor capsid protein)

Beezoo_11

Beezoo_12
(scaffolding protein)

Beezoo_13
(major capsid protein)

Beezoo_14
(head-to-tail adaptor)

Beezoo_15
(head-to-tail stopper)

Beezoo_16

Beezoo_17
(tail terminator)

Beezoo_18

Beezoo_19

Beezoo_20

Beezoo_21
(tape measure protein)

Beezoo_22
(minor tail protein)

Beezoo_23
(minor tail protein)

Beezoo_24
(minor tail protein)

Beezoo_25
(minor tail protein)

Beezoo_26
(minor tail protein)

Beezoo_27
(minor tail protein)

Beezoo_28
(minor tail protein)

Beezoo_29

Beezoo_30
(lysin A)

Beezoo_31
(lysin B)

Beezoo_32
(holin)

Beezoo_33

Beezoo_34

Beezoo_35
(MRE11 double-strand break endo/exonuclease)

Beezoo_36

Beezoo_37

Beezoo_38

Beezoo_39

Beezoo_40
(queuine tRNA-ribosyltransferase)

Beezoo_41

Beezoo_42

Beezoo_43
(tyrosine integrase)

Beezoo_44

Beezoo_45
(immunity repressor)

Beezoo_46
(helix-turn-helix DNA binding protein)

Beezoo_47
(excise)

Beezoo_48

Beezoo_49

Beezoo_50

Beezoo_51
(MerR-like helix-turn-helix DNA binding domain protein)

Beezoo_52
(WhiB family transcription factor)

Beezoo_53

Beezoo_54

Beezoo_55

Beezoo_56

Beezoo_57

Beezoo_58

Beezoo_59
(DnaE-like DNA polymerase III)

Beezoo_60

Beezoo_61

Beezoo_62

Beezoo_63
(Cas4 family endonuclease)

Beezoo_64

Beezoo_65

Beezoo_66

Beezoo_67

Beezoo_68
(NrdH-like glutaredoxin)

Beezoo_69

Beezoo_70
(HNH endonuclease)

Beezoo_71
(DNA primase/helicase)

Beezoo_72

Beezoo_73
(RusA-like resolvase)

Beezoo_74

Beezoo_75

Beezoo_76

Beezoo_77

Beezoo_78

Beezoo_79

Beezoo_80

Beezoo_81

Beezoo_82

Beezoo_83

Beezoo_84

Beezoo_85

Beezoo_86

Beezoo_87

Beezoo_88
(RtcB-like RNA ligase)

Beezoo_89

Beezoo_90

Beezoo_91

Beezoo_92

Beezoo_93

Beezoo_94
(HicB-like antitoxin)

Beezoo_95
(HicA-like toxin)

Beezoo_96

Beezoo_97

Beezoo_98

Beezoo_99

Beezoo_100
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH371113
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUbeezooBZZ
SEA Lysate Titer 2.7 x 10^11 pfu/mL
Date of SEA Lysate Titering Nov 3, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# B3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload