The Actinobacteriophage Database

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Detailed Information for Phage LilPharaoh
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Justin Miller
Year Found	2016
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	35°C
GPS Coordinates	34.0665 N, 118.447833 W Map
Discovery Notes	LilPharaoh was isolated from a dry soil sample with low sunlight in an urban environment, collected on 3/30/16. Isolation and characterization was done by Teams Phage Advice (Vasileios Ragkousis, Rohan Luhar, Justin Miller, Alysha Salbato) and PowerPhagers (Hayley Ennis, Joseph Gaballa, Krishna Govindaraju, Stephanie Orchanian). UA: Emily Xu. TA: Emma Goodwin. Instructors: Dr. Francie Mercer and Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Annotation QC performed by Andrew Lund.
Naming Notes	A member of the team traveled to Cairo, Egypt and observed a hieroglyphic that represented a tailed phage scribed on a pyramid.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 26, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	1633
Genome length (bp)	56167
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CTCGGGGGCAT
GC Content	67.1%
Sequencing Notes	at position 55987 bp 30% of reads contain a T instead on consensus G
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Lysogeny Notes	Clear plaques, but contains integrase and immunity repressor.
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Atiba (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) BGlluviae (K1) Biglebops (K1) Capricorn (K1) Chris (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Deby (K1) DrHayes (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Inky (K1) JAWS (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) MarkPhew (K1) Mdavu (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Niklas (K1) Oscar (K1) Peanam (K1) Prithvi (K1) Ramen (K1) Rapunzel97 (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) Slimphazie (K1) Spock (K1) Sulley (K1) Tachez (K1) TreyKay (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) MacnCheese (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) Boilgate (K4) Chancellor (K4) Cheetobro (K4) Fionnbharth (K4) JF1 (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) Patt (K4) Reptar3000 (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) AlleyCat (K5) Collard (K5) Edugator (K5) Gengar (K5) Guillsminger (K5) Kratio (K5) Larva (K5) Leston (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Rando14 (K5) Thyatira (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Hammy (K6) Krueger (K6) Marshawn (K6) PhelpsODU (K6) Phrank (K6) SirPhilip (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Clear and round 2.3 mm plaques
Morphotype	Siphoviridae
Number of Genes	78
Has been Phamerated?	Yes
Gene List	Click to View LilPharaoh_1 LilPharaoh_2 LilPharaoh_3 (terminase small subunit) LilPharaoh_4 (terminase large subunit) LilPharaoh_5 (portal protein) LilPharaoh_6 (capsid maturation protease) LilPharaoh_7 LilPharaoh_8 (scaffolding protein) LilPharaoh_9 (major capsid protein) LilPharaoh_10 (head-to-tail adaptor) LilPharaoh_11 (head-to-tail stopper) LilPharaoh_12 LilPharaoh_13 (tail terminator) LilPharaoh_14 (major tail protein) LilPharaoh_15 (tail assembly chaperone) LilPharaoh_16 (tail assembly chaperone) LilPharaoh_17 (tapemeasure protein) LilPharaoh_18 (minor tail protein) LilPharaoh_19 (minor tail protein) LilPharaoh_20 (minor tail protein) LilPharaoh_21 (minor tail protein) LilPharaoh_22 (minor tail protein) LilPharaoh_23 (minor tail protein) LilPharaoh_24 (minor tail protein) LilPharaoh_25 LilPharaoh_26 (lysin A) LilPharaoh_27 (lysin B) LilPharaoh_28 (holin) LilPharaoh_29 LilPharaoh_30 LilPharaoh_31 (exonuclease) LilPharaoh_32 (helix-turn-helix DNA binding domain protein) LilPharaoh_33 LilPharaoh_34 LilPharaoh_35 (queuine tRNA-ribosyltransferase) LilPharaoh_36 (integrase) LilPharaoh_37 (membrane protein) LilPharaoh_38 (immunity repressor) LilPharaoh_39 (CRO protein) LilPharaoh_40 (excise) LilPharaoh_41 LilPharaoh_42 LilPharaoh_43 LilPharaoh_44 LilPharaoh_45 (helix-turn-helix DNA binding domain protein) LilPharaoh_46 (WhiB family transcription factor) LilPharaoh_47 LilPharaoh_48 LilPharaoh_49 (DNA polymerase III subunit) LilPharaoh_50 (exonuclease) LilPharaoh_51 LilPharaoh_52 LilPharaoh_53 LilPharaoh_54 LilPharaoh_55 LilPharaoh_56 (glutaredoxin) LilPharaoh_57 (HNH endonuclease) LilPharaoh_58 (DNA primase/polymerase) LilPharaoh_59 (resolvase) LilPharaoh_60 LilPharaoh_61 (helix-turn-helix DNA binding domain protein) LilPharaoh_62 LilPharaoh_63 LilPharaoh_64 LilPharaoh_65 LilPharaoh_66 (membrane domain protein) LilPharaoh_67 (phosphatase) LilPharaoh_68 (RNA ligase) LilPharaoh_69 LilPharaoh_70 LilPharaoh_71 LilPharaoh_72 LilPharaoh_73 (HicB-like antitoxin) LilPharaoh_74 (HicA-like toxin) LilPharaoh_75 LilPharaoh_76 LilPharaoh_77 LilPharaoh_78 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919518
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	28
Pitt Freezer Box Grid#	E6
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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