Mycobacterium phage LilPharaoh
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Detailed Information for Phage LilPharaoh
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJustin Miller
Year Found2016
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.0665 N, 118.447833 W Map
Discovery NotesLilPharaoh was isolated from a dry soil sample with low sunlight in an urban environment, collected on 3/30/16. Isolation and characterization was done by Teams Phage Advice (Vasileios Ragkousis, Rohan Luhar, Justin Miller, Alysha Salbato) and PowerPhagers (Hayley Ennis, Joseph Gaballa, Krishna Govindaraju, Stephanie Orchanian). UA: Emily Xu. TA: Emma Goodwin. Instructors: Dr. Francie Mercer and Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Annotation QC performed by Andrew Lund.
Naming NotesA member of the team traveled
to Cairo, Egypt and observed a hieroglyphic that represented a tailed phage scribed on a pyramid.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 26, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1633
Genome length (bp)56167
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Sequencing Notesat position 55987 bp 30% of reads contain a T instead on consensus G
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesClear plaques, but contains integrase and immunity repressor.
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear and round 2.3 mm plaques
MorphotypeSiphoviridae
Number of Genes78
Has been Phamerated?Yes
Gene List

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LilPharaoh_1

LilPharaoh_2

LilPharaoh_3

LilPharaoh_4
(terminase)

LilPharaoh_5
(portal protein)

LilPharaoh_6
(MuF-like minor capsid protein)

LilPharaoh_7

LilPharaoh_8
(scaffolding protein)

LilPharaoh_9
(major capsid protein)

LilPharaoh_10
(head-to-tail adaptor)

LilPharaoh_11
(head-to-tail stopper)

LilPharaoh_12

LilPharaoh_13
(tail terminator)

LilPharaoh_14

LilPharaoh_15

LilPharaoh_16

LilPharaoh_17
(tape measure protein)

LilPharaoh_18
(minor tail protein)

LilPharaoh_19
(minor tail protein)

LilPharaoh_20
(minor tail protein)

LilPharaoh_21
(minor tail protein)

LilPharaoh_22
(minor tail protein)

LilPharaoh_23
(minor tail protein)

LilPharaoh_24
(minor tail protein)

LilPharaoh_25

LilPharaoh_26
(lysin A)

LilPharaoh_27
(lysin B)

LilPharaoh_28
(holin)

LilPharaoh_29

LilPharaoh_30

LilPharaoh_31
(MRE11 double-strand break endo/exonuclease)

LilPharaoh_32

LilPharaoh_33

LilPharaoh_34

LilPharaoh_35
(queuine tRNA-ribosyltransferase)

LilPharaoh_36
(tyrosine integrase)

LilPharaoh_37

LilPharaoh_38
(immunity repressor)

LilPharaoh_39
(Cro protein)

LilPharaoh_40
(excise)

LilPharaoh_41

LilPharaoh_42

LilPharaoh_43

LilPharaoh_44

LilPharaoh_45
(MerR-like helix-turn-helix DNA binding domain protein)

LilPharaoh_46
(WhiB family transcription factor)

LilPharaoh_47

LilPharaoh_48

LilPharaoh_49
(DnaE-like DNA polymerase III)

LilPharaoh_50
(Cas4 family endonuclease)

LilPharaoh_51

LilPharaoh_52

LilPharaoh_53

LilPharaoh_54

LilPharaoh_55

LilPharaoh_56
(NrdH-like glutaredoxin)

LilPharaoh_57
(HNH endonuclease)

LilPharaoh_58
(DNA primase/helicase)

LilPharaoh_59
(RusA-like resolvase)

LilPharaoh_60

LilPharaoh_61

LilPharaoh_62

LilPharaoh_63

LilPharaoh_64

LilPharaoh_65

LilPharaoh_66

LilPharaoh_67
(polynucleotide kinase)

LilPharaoh_68
(RtcB-like RNA ligase)

LilPharaoh_69

LilPharaoh_70

LilPharaoh_71

LilPharaoh_72

LilPharaoh_73
(HicB-like antitoxin)

LilPharaoh_74
(HicA-like toxin)

LilPharaoh_75

LilPharaoh_76

LilPharaoh_77

LilPharaoh_78
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919518
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# E6
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload