Mycobacterium phage Chris
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Detailed Information for Phage Chris
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLillia Davenport and Laura McConahy
Year Found2016
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature22°C
GPS Coordinates47.66754 N, 117.400587 W Map
Discovery NotesChris was found in sandy and bark filled soil near a statue of Jesus. It was raining when we collected our soil sample.
Naming NotesWe named our phage Chris because he is a temperate phage and we thought Chris was a mellow name. We also wanted a person name because our phage is a living being.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 16, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1233
Genome length (bp)62067
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content67.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Inky (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionGonzaga University
Annotation StatusIn GenBank
Plaque NotesThe plaques were 3mm in diameter, round, and turbid. They had a clear center and were cloudy on the edges. It took at least 72 hours to see significant plaque growth.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Chris_1

Chris_2

Chris_3

Chris_5

Chris_6
(terminase small subunit)

Chris_7
(terminase large subunit)

Chris_8
(portal protein)

Chris_9
(capsid maturation protease)

Chris_10

Chris_11
(scaffolding protein)

Chris_12
(major capsid protein)

Chris_13
(head-to-tail adaptor)

Chris_14
(head-to-tail stopper)

Chris_15

Chris_16
(tail terminator)

Chris_17
(major tail protein)

Chris_18
(tail assembly chaperone)

Chris_19
(tail assembly chaperone)

Chris_20
(tape measure protein)

Chris_21
(minor tail protein)

Chris_22
(minor tail protein)

Chris_23
(minor tail protein)

Chris_24
(minor tail protein)

Chris_25
(minor tail protein)

Chris_26
(minor tail protein)

Chris_27
(minor tail protein)

Chris_28

Chris_29
(lysin A)

Chris_30
(lysin B)

Chris_31
(holin)

Chris_32

Chris_33

Chris_34

Chris_35
(exonuclease)

Chris_36
(helix-turn-helix DNA binding domain protein)

Chris_37

Chris_38

Chris_39

Chris_40

Chris_41

Chris_42
(queuine tRNA-ribosyltransferase)

Chris_43
(tyrosine integrase)

Chris_44

Chris_45
(immunity repressor)

Chris_46
(Cro protein)

Chris_47
(excise)

Chris_48

Chris_49

Chris_50

Chris_51

Chris_52
(helix-turn-helix DNA binding domain protein)

Chris_53
(WhiB family transcription factor)

Chris_54

Chris_55

Chris_56

Chris_57

Chris_58

Chris_59
(DNA polymerase III subunit)

Chris_60

Chris_61

Chris_62
(exonuclease)

Chris_63

Chris_64

Chris_65

Chris_66

Chris_67

Chris_68

Chris_69
(NrdH-like glutaredoxin)

Chris_70
(HNH endonuclease)

Chris_71
(DNA primase/polymerase)

Chris_72
(RusA-like resolvase)

Chris_73

Chris_74
(helix-turn-helix DNA binding domain protein)

Chris_75

Chris_76

Chris_77

Chris_78

Chris_79
(helix-turn-helix DNA binding domain protein)

Chris_80

Chris_81

Chris_82

Chris_83

Chris_84

Chris_85

Chris_86
(RtcB-like RNA ligase)

Chris_87

Chris_88

Chris_89

Chris_90

Chris_91

Chris_92

Chris_93
(HicB-like antitoxin)

Chris_94
(HicA-like toxin)

Chris_95
(esterase)

Chris_96

Chris_97

Chris_98

Chris_99

Chris_100

Chris_101
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT310860
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 40
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload