The Actinobacteriophage Database | Phage SgtBeansprout

Mycobacterium phage SgtBeansprout

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Detailed Information for Phage SgtBeansprout
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Samantha Mosier
Year Found	2016
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	35°C
GPS Coordinates	34.068242 N, 118.442184 W Map
Discovery Notes	SgtBeansprout was isolated from a moist and oily soil sample (traffic, human/animal hair observed nearby) on 3/30/16. Isolation and characterization was done by Teams Nicholas Phage (Christina Fournier, Christy Kim, Ivonne Romero, Mikael Sanchez) and Agarvated Buns and Burners (Nam Do, Samantha Mosier, Joyce Wang, Sam Wu). Annotation performed by team Plaque It Up (Rina Watanabe, O. Monifa Sawyerr, Carly Pierson.) UA: Emily Xu. TA: Emma Goodwin. Instructors: Dr. Francis Mercer and Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Annotation QC by Andrew Lund.
Naming Notes	SgtBeansprout was so named because under the electron microscope, the phage looked like a bean sprout due to its long striated tail. Also, many capsid heads separated from their tails depicting a field resembling that of war. Therefore, the phage was named SgtBeanSprout.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 7, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Genome length (bp)	56439
Character of genome ends	Unknown
Overhang Length	11 bases
Overhang Sequence	CTCGGGGGCAT
GC Content	67.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	K
Subcluster	K1
Cluster Life Cycle	Temperate
Lysogeny Notes	No evidence of lysogeny.
Other Cluster Members	Click to View ActinUp (K1) Adephagia (K1) Adonis (K1) Ageofdapage (K1) AlishaPH (K1) Amelie (K1) Anaya (K1) Angelica (K1) Apocalypse (K1) Asayake (K1) Atiba (K1) BaghaKamala (K1) BarrelRoll (K1) BEEST (K1) Beezoo (K1) Bella96 (K1) Belladonna (K1) BGlluviae (K1) Biglebops (K1) Blizzard (K1) Boiiii (K1) Capricorn (K1) CaseJules (K1) CheetoDust (K1) Chris (K1) CREW (K1) CrimD (K1) Curiosium (K1) Dalmuri (K1) Deby (K1) Devera (K1) Dole (K1) DrHayes (K1) Durfee (K1) Efra2 (K1) Emerson (K1) Enkosi (K1) Ganymede (K1) Geraldini (K1) Guanica15 (K1) HedwigODU (K1) Homura (K1) Hyperbowlee (K1) Illumine (K1) Inky (K1) Jarvi (K1) JAWS (K1) Jayhawk (K1) Jeckyll (K1) Joy99 (K1) KiSi (K1) LastHope (K1) LaterM (K1) LeMond (K1) LilPharaoh (K1) LindNT (K1) Llorens (K1) Lunahalos (K1) MarkPhew (K1) Mdavu (K1) MeaningOfLife (K1) Megsy (K1) Murucutumbu (K1) Mynx (K1) Nibb (K1) Nikao (K1) Niklas (K1) Nutello (K1) Oscar (K1) Padfoot (K1) Padpat (K1) Peanam (K1) Peel (K1) Piatt (K1) Pokerus (K1) Prithvi (K1) QuincyRose (K1) Ramen (K1) Rapunzel97 (K1) Rosmarinus (K1) SamuelLPlaqson (K1) Scarlett (K1) SgtBeansprout (K1) Shaobing (K1) ShiaSurprise (K1) Slimphazie (K1) Spock (K1) Stinson (K1) Sulley (K1) Tachez (K1) TaiwanKao (K1) TiniBug (K1) Tiri (K1) Topper (K1) TreyKay (K1) TruffulaTree (K1) Twitch (K1) Urkel (K1) Validus (K1) Veliki (K1) YoureAdopted (K1) Yunkel11 (K1) Zabiza (K1) Zavala (K1) BoostSeason (K2) DismalFunk (K2) DismalStressor (K2) Findley (K2) Marcoliusprime (K2) Milly (K2) Mufasa (K2) Strobilo (K2) TM4 (K2) ZoeJ (K2) Hurricane (K3) Keshu (K3) MacnCheese (K3) Pharb (K3) Pixie (K3) ShedlockHolmes (K3) TBond007 (K3) TribleTrouble (K3) Chancellor (K4) Cheetobro (K4) Eponine (K4) Fionnbharth (K4) JF1 (K4) Juliette (K4) Malthus (K4) MissDaisy (K4) Mitti (K4) OmniCritical (K4) Patt (K4) Reptar3000 (K4) Ruthiejr (K4) SamScheppers (K4) Slarp (K4) Taquito (K4) Wintermute (K4) Agent47 (K5) AlleyCat (K5) Collard (K5) Dadosky (K5) Edugator (K5) Feyre (K5) Gengar (K5) Guillsminger (K5) Heftyboy (K5) InvictusManeo (K5) Kratio (K5) Larva (K5) Leston (K5) Miryou (K5) OkiRoe (K5) Omnicron (K5) Paola (K5) Psycho (K5) Rando14 (K5) SoSeph (K5) Thyatira (K5) Waterfoul (K5) Amgine (K6) Amohnition (K6) Bryler (K6) Cain (K6) DarthP (K6) Ekdilam (K6) Ellie (K6) Fefferhead (K6) Hammy (K6) Krueger (K6) Marshawn (K6) PhelpsODU (K6) Phrank (K6) Shadow1 (K6) SirPhilip (K6) Sunflower1121 (K6) Syra333 (K6) TClif (K6) Tierra (K6) Unicorn (K6) Ximenita (K6) Yuna (K6) Aminay (K7) Boilgate (K8)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Plaque Notes	Clear and round plaques, 4.615±0.95mm in diameter
Morphotype	Siphoviridae
Number of Genes	78
Has been Phamerated?	Yes
Gene List	Click to View SgtBeansprout_1 SgtBeansprout_2 SgtBeansprout_3 SgtBeansprout_4 (terminase) SgtBeansprout_5 (portal protein) SgtBeansprout_6 (MuF-like minor capsid protein) SgtBeansprout_7 SgtBeansprout_8 (scaffolding protein) SgtBeansprout_9 (major capsid protein) SgtBeansprout_10 (head-to-tail adaptor) SgtBeansprout_11 (head-to-tail stopper) SgtBeansprout_12 SgtBeansprout_13 (tail terminator) SgtBeansprout_14 SgtBeansprout_15 SgtBeansprout_16 SgtBeansprout_17 (tape measure protein) SgtBeansprout_18 (minor tail protein) SgtBeansprout_19 (minor tail protein) SgtBeansprout_20 (minor tail protein) SgtBeansprout_21 (minor tail protein) SgtBeansprout_22 (minor tail protein) SgtBeansprout_23 (minor tail protein) SgtBeansprout_24 (minor tail protein) SgtBeansprout_25 SgtBeansprout_26 (lysin A) SgtBeansprout_27 (lysin B) SgtBeansprout_28 (holin) SgtBeansprout_29 SgtBeansprout_30 SgtBeansprout_31 (MRE11 double-strand break endo/exonuclease) SgtBeansprout_32 SgtBeansprout_33 SgtBeansprout_34 SgtBeansprout_35 (queuine tRNA-ribosyltransferase) SgtBeansprout_36 (tyrosine integrase) SgtBeansprout_37 SgtBeansprout_38 (immunity repressor) SgtBeansprout_39 (Cro protein) SgtBeansprout_40 (excise) SgtBeansprout_41 SgtBeansprout_42 SgtBeansprout_43 SgtBeansprout_44 SgtBeansprout_45 (MerR-like helix-turn-helix DNA binding domain protein) SgtBeansprout_46 (WhiB family transcription factor) SgtBeansprout_47 SgtBeansprout_48 SgtBeansprout_49 (DnaE-like DNA polymerase III) SgtBeansprout_50 (Cas4 family endonuclease) SgtBeansprout_51 SgtBeansprout_52 SgtBeansprout_53 SgtBeansprout_54 SgtBeansprout_55 SgtBeansprout_56 (NrdH-like glutaredoxin) SgtBeansprout_57 (HNH endonuclease) SgtBeansprout_58 (DNA primase/helicase) SgtBeansprout_59 (RusA-like resolvase) SgtBeansprout_60 SgtBeansprout_61 SgtBeansprout_62 SgtBeansprout_63 SgtBeansprout_64 SgtBeansprout_65 SgtBeansprout_66 SgtBeansprout_67 (polynucleotide kinase) SgtBeansprout_68 (RtcB-like RNA ligase) SgtBeansprout_69 SgtBeansprout_70 SgtBeansprout_71 SgtBeansprout_72 SgtBeansprout_73 (HicB-like antitoxin) SgtBeansprout_74 (HicA-like toxin) SgtBeansprout_75 SgtBeansprout_76 SgtBeansprout_77 SgtBeansprout_78 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH020245
Refseq Number	None yet
Published in Paper	Gaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley.
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	28
Pitt Freezer Box Grid#	E5
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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