Mycobacterium phage SgtBeansprout
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Detailed Information for Phage SgtBeansprout
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySamantha Mosier
Year Found2016
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature35°C
GPS Coordinates34.068242 N, 118.442184 W Map
Discovery NotesSgtBeansprout was isolated from a moist and oily soil sample (traffic, human/animal hair observed nearby) on 3/30/16. Isolation and characterization was done by Teams Nicholas Phage (Christina Fournier, Christy Kim, Ivonne Romero, Mikael Sanchez) and Agarvated Buns and Burners (Nam Do, Samantha Mosier, Joyce Wang, Sam Wu). Annotation performed by team Plaque It Up (Rina Watanabe, O. Monifa Sawyerr, Carly Pierson.) UA: Emily Xu. TA: Emma Goodwin. Instructors: Dr. Francis Mercer and Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Annotation QC by Andrew Lund.
Naming NotesSgtBeansprout was so named because under the electron microscope, the phage looked like a bean sprout due to its long striated tail. Also, many capsid heads separated from their tails depicting a field resembling that of war. Therefore, the phage was named SgtBeanSprout.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 7, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)56439
Character of genome endsUnknown
Overhang Length11 bases
Overhang SequenceCTCGGGGGCAT
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Lysogeny NotesNo evidence of lysogeny.
Other Cluster Members

Click to View

ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesClear and round plaques, 4.615±0.95mm in diameter
MorphotypeSiphoviridae
Number of Genes78
Has been Phamerated?Yes
Gene List

Click to View

SgtBeansprout_1

SgtBeansprout_2

SgtBeansprout_3

SgtBeansprout_4
(terminase)

SgtBeansprout_5
(portal protein)

SgtBeansprout_6
(MuF-like minor capsid protein)

SgtBeansprout_7

SgtBeansprout_8
(scaffolding protein)

SgtBeansprout_9
(major capsid protein)

SgtBeansprout_10
(head-to-tail adaptor)

SgtBeansprout_11
(head-to-tail stopper)

SgtBeansprout_12

SgtBeansprout_13
(tail terminator)

SgtBeansprout_14

SgtBeansprout_15

SgtBeansprout_16

SgtBeansprout_17
(tape measure protein)

SgtBeansprout_18
(minor tail protein)

SgtBeansprout_19
(minor tail protein)

SgtBeansprout_20
(minor tail protein)

SgtBeansprout_21
(minor tail protein)

SgtBeansprout_22
(minor tail protein)

SgtBeansprout_23
(minor tail protein)

SgtBeansprout_24
(minor tail protein)

SgtBeansprout_25

SgtBeansprout_26
(lysin A)

SgtBeansprout_27
(lysin B)

SgtBeansprout_28
(holin)

SgtBeansprout_29

SgtBeansprout_30

SgtBeansprout_31
(MRE11 double-strand break endo/exonuclease)

SgtBeansprout_32

SgtBeansprout_33

SgtBeansprout_34

SgtBeansprout_35
(queuine tRNA-ribosyltransferase)

SgtBeansprout_36
(tyrosine integrase)

SgtBeansprout_37

SgtBeansprout_38
(immunity repressor)

SgtBeansprout_39
(Cro protein)

SgtBeansprout_40
(excise)

SgtBeansprout_41

SgtBeansprout_42

SgtBeansprout_43

SgtBeansprout_44

SgtBeansprout_45
(MerR-like helix-turn-helix DNA binding domain protein)

SgtBeansprout_46
(WhiB family transcription factor)

SgtBeansprout_47

SgtBeansprout_48

SgtBeansprout_49
(DnaE-like DNA polymerase III)

SgtBeansprout_50
(Cas4 family endonuclease)

SgtBeansprout_51

SgtBeansprout_52

SgtBeansprout_53

SgtBeansprout_54

SgtBeansprout_55

SgtBeansprout_56
(NrdH-like glutaredoxin)

SgtBeansprout_57
(HNH endonuclease)

SgtBeansprout_58
(DNA primase/helicase)

SgtBeansprout_59
(RusA-like resolvase)

SgtBeansprout_60

SgtBeansprout_61

SgtBeansprout_62

SgtBeansprout_63

SgtBeansprout_64

SgtBeansprout_65

SgtBeansprout_66

SgtBeansprout_67
(polynucleotide kinase)

SgtBeansprout_68
(RtcB-like RNA ligase)

SgtBeansprout_69

SgtBeansprout_70

SgtBeansprout_71

SgtBeansprout_72

SgtBeansprout_73
(HicB-like antitoxin)

SgtBeansprout_74
(HicA-like toxin)

SgtBeansprout_75

SgtBeansprout_76

SgtBeansprout_77

SgtBeansprout_78
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH020245
Refseq NumberNone yet
Published in PaperGaballa JM 2019, Microbiology Resource Announcements: Genome Sequences of Cluster K Mycobacteriophages Deby, LaterM, LilPharaoh, Paola, SgtBeansprout, and Sulley.
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# E5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload