Mycobacterium phage Mitti
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Detailed Information for Phage Mitti
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMaryam Athar Khan
Year Found2016
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.604655 N, 75.374691 W Map
Discovery NotesThis phage was isolated from a potted, money plant at room temperature. The soil in the plant was fertilized soil obtained from a departmental store.
Naming NotesMitti in Urdu means soil. Seeing as this phage was isolated from a soil sample, it was fitting to name it after its home and my home.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 22, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1815
Genome length (bp)57895
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGCGGCCAT
GC Content68.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK4
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques formed are clear with turbid rings surrounding the plaques. The plaques diameter varies between 2-4 mm.
MorphotypeSiphoviridae
Number of Genes94
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Mitti_1

Mitti_2

Mitti_3

Mitti_4

Mitti_5

Mitti_6

Mitti_7

Mitti_8

Mitti_9
(terminase)

Mitti_10
(portal protein)

Mitti_11
(MuF-like minor capsid protein)

Mitti_12

Mitti_13
(scaffolding protein)

Mitti_14
(major capsid protein)

Mitti_15
(head-to-tail adaptor)

Mitti_16
(head-to-tail stopper)

Mitti_17

Mitti_18
(tail terminator)

Mitti_19
(major tail protein)

Mitti_20
(tail assembly chaperone)

Mitti_21
(tail assembly chaperone)

Mitti_22
(tape measure protein)

Mitti_23
(minor tail protein)

Mitti_24
(minor tail protein)

Mitti_25
(minor tail protein)

Mitti_26
(minor tail protein)

Mitti_27

Mitti_28
(minor tail protein)

Mitti_29

Mitti_30

Mitti_31
(lysin A)

Mitti_32
(lysin B)

Mitti_33
(holin)

Mitti_34

Mitti_35

Mitti_36
(MRE11 double-strand break endo/exonuclease)

Mitti_37

Mitti_38 — tRNA
(tRNA-Lys (ttt))

Mitti_39
(LamD-like)

Mitti_40

Mitti_41

Mitti_42

Mitti_43

Mitti_44

Mitti_45
(tyrosine integrase)

Mitti_46

Mitti_47
(immunity repressor)

Mitti_48
(Cro protein)

Mitti_49
(excise)

Mitti_50

Mitti_51

Mitti_52

Mitti_53

Mitti_54

Mitti_55

Mitti_56

Mitti_57

Mitti_58

Mitti_59

Mitti_60

Mitti_61

Mitti_62
(DnaQ-like DNA polymerase III subunit)

Mitti_63

Mitti_64
(Cas4 family exonuclease)

Mitti_65

Mitti_66

Mitti_67

Mitti_68

Mitti_69

Mitti_70
(NrdH-like glutaredoxin)

Mitti_71
(methyltransferase)

Mitti_72

Mitti_73
(HNH endonuclease)

Mitti_74
(DNA primase)

Mitti_75

Mitti_76
(HNH endonuclease)

Mitti_77

Mitti_78

Mitti_79

Mitti_80

Mitti_81
(SprT-like protease)

Mitti_82

Mitti_83

Mitti_84

Mitti_85

Mitti_86

Mitti_87

Mitti_88

Mitti_89

Mitti_90

Mitti_91

Mitti_92

Mitti_93

Mitti_94
(HNH endonuclease)

Mitti_95
(O-methyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY087992
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator Maryam Athar Khan
SEA Lysate Titer 5.4x10^10
Date of SEA Lysate Titering Apr 13, 2016
Pitt Freezer Box# 35
Pitt Freezer Box Grid# C1
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload