Detailed Information for Phage Twitch |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Lauren Sellers |
Year Found | 2013 |
Location Found | Holland, MI USA |
Finding Institution | Hope College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 42.798056 N, 86.093889 W Map |
Discovery Notes | The soil sample from which this phage was extracted was taken from a stream bank 2 meters from the water, and 3 meters from a concrete sidewalk. It was taken from a depth of 5 centimeters below the surface, and was dry in consistency. The temperature was 25 degrees Celsius, with a 79% humidity. It was collected on the 28th of August, 2013. |
Naming Notes | "Twitch" seemed like an appropriate name for a mycobacteriophage. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Aug 9, 2019 |
Sequencing Facility | Hope College |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 668 |
Genome length (bp) | 59711 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | CTCGTAGGCAT |
GC Content | 66.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Benedictine University |
Annotation Status | In GenBank |
Plaque Notes | Phage "Twitch" displays a clear plaque with defined boarders. It is not partial to growing at 30 degrees Celsius or 37 degrees Celsius. The plaques are very consistently 5 millimeters in diameter. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MW712722 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.6x10^11 |
Pitt Freezer Box# |
5 |
Pitt Freezer Box Grid# |
C6 |
Available Files |
Plaque Picture | Download |
GenBank File for Phamerator | Download |