Mycobacterium phage Twitch
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Detailed Information for Phage Twitch
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLauren Sellers
Year Found2013
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.798056 N, 86.093889 W Map
Discovery NotesThe soil sample from which this phage was extracted was taken from a stream bank 2 meters from the water, and 3 meters from a concrete sidewalk. It was taken from a depth of 5 centimeters below the surface, and was dry in consistency. The temperature was 25 degrees Celsius, with a 79% humidity. It was collected on the 28th of August, 2013.
Naming Notes"Twitch" seemed like an appropriate name for a mycobacteriophage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 9, 2019
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage668
Genome length (bp)59711
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TreyKay (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

Boilgate (K4)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

AlleyCat (K5)

Collard (K5)

Edugator (K5)

Gengar (K5)

Guillsminger (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

SirPhilip (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Annotating InstitutionBenedictine University
Annotation StatusIn GenBank
Plaque NotesPhage "Twitch" displays a clear plaque with defined boarders. It is not partial to growing at 30 degrees Celsius or 37 degrees Celsius. The plaques are very consistently 5 millimeters in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Twitch_1

Twitch_2

Twitch_3
(ATPase domain)

Twitch_4

Twitch_6

Twitch_7

Twitch_8
(terminase)

Twitch_9
(portal protein)

Twitch_10
(MuF-like minor capsid protein)

Twitch_11

Twitch_12
(scaffolding protein)

Twitch_13
(major capsid protein)

Twitch_14
(head to tail adaptor)

Twitch_15
(head to tail stopper)

Twitch_16

Twitch_17
(tail terminator)

Twitch_18
(major tail protein)

Twitch_19
(tail assembly chaperone)

Twitch_20
(tail assembly chaperone)

Twitch_21
(tape measure protein)

Twitch_22
(minor tail protein)

Twitch_23
(minor tail protein)

Twitch_24
(Hyothetical Protein)

Twitch_25
(minor tail protein)

Twitch_26

Twitch_27
(minor tail protein)

Twitch_28
(minor tail protein)

Twitch_29

Twitch_30
(lysin A)

Twitch_31
(lysin B)

Twitch_32
(holin)

Twitch_33

Twitch_34

Twitch_35
(exonuclease)

Twitch_36
(helix-turn-helix DNA binding protein)

Twitch_37

Twitch_38

Twitch_39

Twitch_40

Twitch_41
(tyrosine integrase)

Twitch_42

Twitch_43
(immunity repressor)

Twitch_44
(helix-turn-helix DNA binding protein)

Twitch_45
(excise)

Twitch_46

Twitch_47

Twitch_48

Twitch_49
(helix-turn-helix DNA binding protein)

Twitch_50
(WhiB family transcription factor)

Twitch_51

Twitch_52

Twitch_53

Twitch_54

Twitch_55

Twitch_56

Twitch_57
(DnaQ-like (DNA polymerase III subunit))

Twitch_58

Twitch_59

Twitch_60

Twitch_61
(exonuclease)

Twitch_62

Twitch_63

Twitch_64

Twitch_65

Twitch_66
(NrdH glutaredoxin)

Twitch_67

Twitch_68
(HNH endonuclease)

Twitch_69
(DNA primase)

Twitch_70

Twitch_71
(RusA-like resolvase (endonuclease))

Twitch_72

Twitch_73

Twitch_74

Twitch_75

Twitch_76
(SprT-like protease)

Twitch_77

Twitch_78

Twitch_79
(helix-turn helix DNA binding protein)

Twitch_80

Twitch_81

Twitch_82

Twitch_83

Twitch_84

Twitch_85
(RtcB-like RNA ligase)

Twitch_86

Twitch_87

Twitch_88

Twitch_89

Twitch_90

Twitch_91
(antitoxin in toxin/antitoxin system)

Twitch_92
(toxin in toxin/antitoxin system, HicA-like)

Twitch_93

Twitch_94

Twitch_95

Twitch_96

Twitch_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW712722
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.6x10^11
Pitt Freezer Box# 5
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload