Mycobacterium phage TribleTrouble
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Detailed Information for Phage TribleTrouble
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySofia Sileo
Year Found2022
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986335 N, 86.454495 W Map
Discovery NotesThe soil was collected by a tree near a common walkway on Western Kentucky’s Campus 2 inches below the surface of the soil. It was chosen due the large amount of dead leaves littering the surface. Additionally, the soil was dry and crumbly.
Naming NotesThe phage is named after my mom using her maiden name, Trible.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe phage produces turbid plaques. Turbid plaques indicates my phage goes through the lysogenic cycle (integrates its DNA into the host's genome). The plaques are consistently 1 to 2 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.0 * 10^7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload