Mycobacterium phage TribleTrouble
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Detailed Information for Phage TribleTrouble
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySofia Sileo
Year Found2022
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986335 N, 86.454495 W Map
Discovery NotesThe soil was collected by a tree near a common walkway on Western Kentucky’s Campus 2 inches below the surface of the soil. It was chosen due the large amount of dead leaves littering the surface. Additionally, the soil was dry and crumbly.
Naming NotesThe phage is named after my mom using her maiden name, Trible.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1913
Genome length (bp)61665
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGATGGCAT
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK3
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesThe phage produces turbid plaques. Turbid plaques indicates my phage goes through the lysogenic cycle (integrates its DNA into the host's genome). The plaques are consistently 1 to 2 mm in diameter.
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

TribleTrouble_1

TribleTrouble_2

TribleTrouble_3

TribleTrouble_4

TribleTrouble_5

TribleTrouble_6
(terminase)

TribleTrouble_7
(portal protein)

TribleTrouble_8
(minor capsid protein)

TribleTrouble_9

TribleTrouble_10
(scaffolding protein)

TribleTrouble_11
(major capsid protein)

TribleTrouble_12
(head-to-tail adaptor)

TribleTrouble_13

TribleTrouble_14

TribleTrouble_15
(tail terminator)

TribleTrouble_16

TribleTrouble_17
(tail assembly chaperone)

TribleTrouble_18
(tail assembly chaperone)

TribleTrouble_19
(tape measure protein)

TribleTrouble_20
(minor tail protein)

TribleTrouble_21
(minor tail protein)

TribleTrouble_22
(minor tail protein)

TribleTrouble_23
(minor tail protein)

TribleTrouble_24
(minor tail protein)

TribleTrouble_25
(minor tail protein)

TribleTrouble_26

TribleTrouble_27
(minor tail protein)

TribleTrouble_28

TribleTrouble_29
(lysin A)

TribleTrouble_30
(lysin B)

TribleTrouble_31
(holin)

TribleTrouble_32

TribleTrouble_33

TribleTrouble_34
(metallophosphoesterase)

TribleTrouble_35

TribleTrouble_36

TribleTrouble_37
(helix-turn-helix DNA binding domain)

TribleTrouble_38

TribleTrouble_39

TribleTrouble_40
(tyrosine integrase)

TribleTrouble_41

TribleTrouble_42

TribleTrouble_43
(immunity repressor)

TribleTrouble_44
(Cro (control of repressor)

TribleTrouble_45
(excise)

TribleTrouble_46

TribleTrouble_47

TribleTrouble_48

TribleTrouble_49

TribleTrouble_50
(WhiB family transcription factor)

TribleTrouble_51

TribleTrouble_52

TribleTrouble_53

TribleTrouble_54

TribleTrouble_55
(helix-turn-helix DNA binding domain)

TribleTrouble_56
(exonuclease)

TribleTrouble_57

TribleTrouble_58

TribleTrouble_59

TribleTrouble_60
(Cas4 exonuclease)

TribleTrouble_61

TribleTrouble_62

TribleTrouble_63

TribleTrouble_64

TribleTrouble_65

TribleTrouble_66

TribleTrouble_67

TribleTrouble_68
(NrdH-like glutaredoxin)

TribleTrouble_69
(HNH endonuclease)

TribleTrouble_70
(DNA primase/helicase)

TribleTrouble_71

TribleTrouble_72
(RusA-like resolvase (endonuclease))

TribleTrouble_73

TribleTrouble_74

TribleTrouble_75
(DNA binding protein)

TribleTrouble_76

TribleTrouble_77

TribleTrouble_78
(SprT-like protease)

TribleTrouble_79

TribleTrouble_80

TribleTrouble_81

TribleTrouble_82

TribleTrouble_83

TribleTrouble_84

TribleTrouble_85

TribleTrouble_86
(rtcB-like RNA ligase)

TribleTrouble_87

TribleTrouble_88

TribleTrouble_89

TribleTrouble_90

TribleTrouble_91

TribleTrouble_92

TribleTrouble_93

TribleTrouble_94

TribleTrouble_95

TribleTrouble_96

TribleTrouble_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR475294
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.0 * 10^7
Pitt Freezer Box# 157
Pitt Freezer Box Grid# H10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload