Mycobacterium phage Bella96
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Detailed Information for Phage Bella96
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHaley Fischman
Year Found2014
Location FoundGrand Ledge, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates42.711111 N, 84.777778 W Map
Discovery NotesDiscovered in a week compost pile in mid-Michigan
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage344
Genome length (bp)60746
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCGTAGGCAT
GC Content66.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK1
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Dartin (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

McMater (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Richo (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TingHuaYa (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

Torres (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Kraw (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall turbid plaques, about 3mm in diameter at 24 hour incubation time. Produced a mesa after 3 days incubation time on a spot test.
Does not produce plaques at 42 degrees Celsius, after 24 hour incubation.
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Bella96_1

Bella96_2

Bella96_3

Bella96_4

Bella96_5 — tRNA
(tRNA-Trp(cca))

Bella96_6

Bella96_7

Bella96_8
(terminase)

Bella96_9
(portal protein)

Bella96_10
(MuF-like minor capsid protein)

Bella96_11

Bella96_12
(scaffolding protein)

Bella96_13
(major capsid protein)

Bella96_14
(head-to-tail adaptor)

Bella96_15
(head-to-tail stopper)

Bella96_16

Bella96_17
(tail terminator)

Bella96_18

Bella96_19

Bella96_20

Bella96_21
(tape measure protein)

Bella96_22
(minor tail protein)

Bella96_23
(minor tail protein)

Bella96_24
(minor tail protein)

Bella96_25
(minor tail protein)

Bella96_26

Bella96_27
(minor tail protein)

Bella96_28

Bella96_29

Bella96_30
(lysin A)

Bella96_31
(lysin B)

Bella96_32
(holin)

Bella96_33

Bella96_34

Bella96_35

Bella96_36

Bella96_37

Bella96_38

Bella96_39

Bella96_40
(queuine tRNA-ribosyltransferase)

Bella96_41

Bella96_42
(tyrosine integrase)

Bella96_43

Bella96_44
(immunity repressor)

Bella96_45
(helix-turn-helix DNA binding protein)

Bella96_46
(excise)

Bella96_47

Bella96_48

Bella96_49

Bella96_50
(MerR-like helix-turn-helix DNA binding domain protein)

Bella96_51
(WhiB family transcription factor)

Bella96_52

Bella96_53

Bella96_54

Bella96_55

Bella96_56

Bella96_57

Bella96_58
(DnaE-like DNA polymerase III)

Bella96_59

Bella96_60

Bella96_61

Bella96_62

Bella96_63

Bella96_64

Bella96_65

Bella96_66

Bella96_67
(NrdH-like glutaredoxin)

Bella96_68

Bella96_69
(DNA primase/helicase)

Bella96_70

Bella96_71

Bella96_72
(RusA-like resolvase)

Bella96_73

Bella96_74

Bella96_75

Bella96_76

Bella96_77
(SprT-like protease)

Bella96_78

Bella96_79

Bella96_80

Bella96_81

Bella96_82

Bella96_83

Bella96_84

Bella96_85

Bella96_86
(RtcB-like RNA ligase)

Bella96_87

Bella96_88

Bella96_89

Bella96_90

Bella96_91

Bella96_92

Bella96_93

Bella96_94

Bella96_95

Bella96_96

Bella96_97

Bella96_98
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF377440
Refseq NumberNone yet
Published in PaperAnders et al. 2017, Genome Announcements: Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.52e10
Pitt Freezer Box# 7
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload